Anti-Chromogranin B Rabbit Monoclonal Antibody for WB, ELISA - P05060

Core Product Specifications and Parameters

Biological Background: Chromogranin B Function and Localization

• Chromogranin B, also designated Secretogranin-1 (SCG1), is a member of the granin family of neuroendocrine secretory proteins.
• It serves as a precursor that can be proteolytically processed to release smaller, potentially bioactive peptides.
• The protein is classified within the chromogranin/secretogranin family, characterized by their acidic nature and presence in secretory granules.
• Subcellular localization is primarily within secretory vesicles, with secretion occurring upon appropriate stimulation.
• Tissue expression is predominantly in the adrenal medulla and pheochromocytoma, and the protein is detectable in cerebrospinal fluid and urine, but absent from liver. Related references: PMID:25326458, PMID:37453717
• Post‑translational modifications include glycosylation, phosphorylation, sulfation, and the formation of disulfide bonds, and it can exist as a proteoglycan.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide within amino acids 550–650 of human Chromogranin B; antibody reactivity may be influenced by epitope accessibility in full‑length or processed forms.
• For Western blotting, consider running full‑length recombinant protein or tissue lysates with known expression to confirm band specificity at the expected molecular weight.
• In ELISA, validate performance with purified antigen or characterized sample matrices to establish optimal coating conditions and detection limits.
• Cross‑reactivity with rat has been observed; however, epitope conservation should be verified in the specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, laboratories, and research institutes with seamless access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For neuroendocrine research and diagnostic development, this Chromogranin B antibody exemplifies our capability to deliver high‑quality reagents for immunodetection workflows. From concept to clinic, CamelBio simplifies procurement of rare and routine raw materials, backed by technical expertise and supply chain reliability.