Anti-Chromogranin A Rabbit Monoclonal Antibody for IF-P, IHC-P, ELISA - P10645

Core Product Specifications and Parameters

Biological Background: Chromogranin A Function and Localization

• Chromogranin A (CgA, also known as pituitary secretory protein I, SP-I) is a member of the granin family of neuroendocrine secretory proteins, encoded by the CHGA gene (Gene ID: 1113).
• It strongly inhibits glucose-induced insulin release from the pancreas, contributing to the regulation of glucose homeostasis.
• CgA acts as a non-competitive nicotinic cholinergic antagonist to inhibit catecholamine release from chromaffin cells and noradrenergic neurons (PubMed:15326220). Related references: PMID:15326220
• It displays broad-spectrum antibacterial activity against Gram-positive bacteria S. aureus and M. luteus, and Gram-negative bacteria E. coli and P. aeruginosa (PubMed:15723172, PubMed:24723458). Related references: PMID:15723172 PMID:24723458
• CgA can induce mast cell migration, degranulation and production of cytokines and chemokines, implicating it in innate immune responses (PubMed:21214543). Related references: PMID:21214543
• Chromogranin A may modulate cardiac function and blood pressure regulation (PubMed:18541522). Related references: PMID:18541522
• In endocrine cells, CgA regulates secretory granule biogenesis by up-regulating protease nexin 1 (SERPINE2) via a cAMP-PKA-SP1 pathway.
• Subcellularly, CgA localizes to secretory vesicles including neuronal dense core vesicles and is secreted; it is detected in brain, cerebrospinal fluid, and urine.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–100) of human Chromogranin A, which is exposed in the mature secreted protein. Consider using a blocking peptide for specificity controls in immunohistochemistry or ELISA.
• For IHC-P, antigen retrieval in citrate buffer may be required; validate with known positive control tissues such as pancreas or adrenal gland.
• For IF-P, optimize fixation and permeabilization conditions for your sample type. Co-staining with a granule marker can confirm localization.
• In ELISA, this antibody may be used as a capture or detection reagent; pairing with a different epitope-specific antibody is recommended for sandwich assays.
• Cross-reactivity with mouse and rat has been reported, but verify performance in the relevant species by pilot experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to validated IVD raw materials, including optimized monoclonal antibodies, antibody pairs, and bulk ancillary reagents for chromogranin A detection. As a comprehensive B2B sourcing partner, CamelBio supports neuroendocrine research, cardiovascular biomarker studies, and infectious disease applications with one-stop procurement from concept to clinic. Whether you need this anti-Chromogranin A rabbit monoclonal antibody for IF-P, IHC-P, or ELISA, or seek additional specificities and formats, our supply capabilities can help accelerate your assay development.