Anti-CHRNG Polyclonal Antibody for WB, ELISA - P07510

Core Product Specifications and Parameters

Biological Background: Acetylcholine receptor subunit gamma Function and Localization

• The CHRNG gene encodes the gamma subunit of the nicotinic acetylcholine receptor (AChR), a ligand-gated ion channel that mediates fast synaptic transmission at the neuromuscular junction.
• Upon acetylcholine binding, the AChR undergoes extensive conformational changes that open the ion-conducting channel, permitting cation influx and depolarizing the postsynaptic membrane.
• CHRNG is a glycoprotein with multiple transmembrane helices; together with other subunits, it forms the central ion channel pore essential for signal transduction.
• The protein features disulfide bonds and a signal peptide, characteristic of membrane-bound receptors, and undergoes alternative splicing to generate distinct isoforms.
• Subcellular localization studies indicate CHRNG is present at the postsynaptic cell membrane and the cell membrane, aligning with its synaptic signaling function.
• Keywords associated with CHRNG include ion transport, ligand-gated ion channel, disease variant, and glycoprotein, highlighting its role in neuromuscular transmission and pathophysiological conditions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 328-517, located in the intracellular domain of CHRNG; thus, the antibody may preferentially detect the denatured protein in Western blotting under reducing conditions.
• For Western blot, consider validating the antibody using lysates from cell lines or tissues known to express CHRNG, such as skeletal muscle or neuronal cells.
• In ELISA, the antibody may recognize native or recombinant CHRNG; optimization of coating buffers and blocking agents is recommended.
• When probing mouse or rat samples, confirm reactivity through initial cross-reactivity testing, as the antibody is predicted to cross-react with these species based on sequence homology.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories streamlined access to high-quality IVD raw materials for neurological targets such as the acetylcholine receptor gamma subunit. Our portfolio includes validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents tailored for ion channel research. Whether you require standard catalog products or custom rare-target sourcing, CamelBio bridges your concept-to-clinic pipeline with reliable and scalable supply solutions.