Anti-CHRNB2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P17787

Core Product Specifications and Parameters

Biological Background: CHRNB2 Function and Localization

• CHRNB2 encodes the neuronal acetylcholine receptor subunit beta-2, an integral membrane protein that assembles with other nAChR subunits to form pentameric ligand-gated cation channels mediating fast excitatory neurotransmission.
• nAChRs containing the beta-2 subunit exhibit high calcium permeability and play crucial roles in synaptic transmission, with channel properties finely tuned by subunit composition, including activation, deactivation and desensitization kinetics, pH sensitivity, and allosteric modulator binding. Related references: PMID:22361591 PMID:27698419 PMID:29720657
• CHRNB2 forms heteropentameric receptors with various alpha subunits, particularly CHRNA2, CHRNA3, CHRNA4 and CHRNA6, as well as accessory subunits CHRNA5 and CHRNB3, generating diverse receptor subtypes with distinct pharmacological and biophysical profiles. Related references: PMID:16835356 PMID:20881005 PMID:22361591
• The (CHRNA4)3:(CHRNB2)2 and (CHRNA4)2:(CHRNB2)3 stoichiometries exhibit differences in unitary conductance, calcium permeability, ACh sensitivity and modulation by divalent cations, underscoring the functional plasticity of beta-2-containing receptors. Related references: PMID:27698419 PMID:29720657 PMID:38454578
• nAChR subtypes containing both CHRNA6 and CHRNB2 are highly sensitive to ACh and nicotine and are predominantly expressed in dopaminergic and norepinephrinergic neurons, where they modulate dopamine and GABA release in response to nicotine.
• CHRNB2 also forms functional receptors with CHRNA7, mainly identified in basal forebrain cholinergic neurons, expanding the repertoire of beta-2-containing nAChRs. Related references: PMID:33239400 PMID:38161283
• The protein is localized to the synaptic cell membrane and cell membrane, consistent with its role in intercellular signaling, and is subject to post-translational modifications including glycosylation and disulfide bond formation.
• Mutations in CHRNB2 are associated with neurological disorders such as epilepsy, highlighting the clinical relevance of this subunit in human disease.

Experimental Guidance and Technical Tips

• For Western blotting, anticipate a specific band near the predicted molecular weight of 57 kDa; validate using appropriate positive and negative control lysates from relevant species (human, mouse, or rat).
• For immunofluorescence/ICC, optimize fixation and permeabilization protocols to preserve membrane protein integrity, and confirm expected membranous staining pattern.
• As the immunogen corresponds to a C-terminal fragment (aa 340-430), the antibody may detect full-length CHRNB2 and potentially some processing variants; cross-reactivity with closely related nAChR subunits should be carefully evaluated.
• Given reported cross-reactivity with mouse and rat, this antibody is a candidate for translational studies, but species-specific reactivity should be verified in your experimental context.

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