Anti-CHRNA7 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P36544

Core Product Specifications and Parameters

Biological Background: CHRNA7 (Alpha-7 nAChR) Function and Localization

• CHRNA7 encodes the alpha-7 subunit of neuronal nicotinic acetylcholine receptors (nAChRs), and alternative splicing produces multiple isoforms; this immunogen is derived from isoform NP_001177384.1 (extracellular domain, aa 23-230).
• CHRNA7 assembles into homopentameric cation channels with high calcium permeability and fast desensitization kinetics, mediating excitatory neurotransmission in the central nervous system. Related references: PMID:31560909, PMID:33735609, PMID:38382524
• It can also form heteropentamers with CHRNB2, primarily in basal forebrain cholinergic neurons, and modulates neurotransmitter release including dopamine, glutamate, and GABA. Related references: PMID:33239400
• CHRNA7 is expressed in non-neuronal cells such as lymphocytes, monocytes, and macrophages, where it regulates inflammatory responses via the cholinergic anti-inflammatory pathway. Related references: PMID:12508119, PMID:16968406, PMID:25259522
• The receptor is localized at the postsynaptic cell membrane, consistent with its role in synaptic transmission.
• CHRNA7 is a glycoprotein containing disulfide bonds, which are critical for proper folding and receptor assembly.
• Predominantly expressed in neuronal cells, with high levels in brain regions such as the hippocampus and cortex, and also detected in macrophages at the protein level. Related references: PMID:8145738, PMID:12508119

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal extracellular domain (aa 23-230) of human CHRNA7, which is highly conserved across human, mouse, and rat, supporting the observed cross-reactivity.
• For Western blot, the predicted molecular weight is ~54 kDa; consider using appropriate protein extraction and blocking conditions to detect the glycosylated mature form.
• In immunohistochemistry and immunocytochemistry, membrane localization is expected; include appropriate controls and optimize antigen retrieval for paraffin-embedded tissues.
• ELISA can be used for quantitative detection; ensure coating conditions are suitable for the recombinant immunogen or native protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive, one-stop sourcing platform for IVD raw materials, simplifying development from concept to clinic. For neuroscience and immunology research, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. Need affinity-purified anti-CHRNA7 antibodies in large quantities or assistance with custom reagent sourcing? Our team supports diagnostic manufacturers with reliable, scalable supply solutions backed by technical and regulatory expertise.