Anti-CHRNA5 Rabbit Polyclonal Antibody for WB and ELISA - P30532

Core Product Specifications and Parameters

Biological Background: CHRNA5 Function and Localization

• CHRNA5 (Neuronal acetylcholine receptor subunit alpha-5) is an accessory subunit of neuronal nicotinic acetylcholine receptors (nAChRs), which are pentameric ligand-gated cation channels mediating fast synaptic transmission. Related references: PMID:8663494 PMID:20881005
• It modulates receptor properties including activation kinetics, sensitivity to acetylcholine and nicotine, and calcium permeability, but only when co-assembled with beta subunits such as CHRNB2 or CHRNB4. Related references: PMID:8663494
• The alpha-5 subunit has been strongly implicated in nicotine addiction, with genetic variations in CHRNA5 associated with smoking behavior and lung cancer risk. Related references: PMID:20881005
• It is localized to the synaptic cell membrane and cell membrane, consistent with its role in synaptic transmission.
• Post-translational modifications include disulfide bonds and glycosylation, typical of nicotinic receptor subunits, which may affect receptor assembly and function.
• Keywords associated with CHRNA5 include cell membrane, ion channel, ligand-gated ion channel, synapse, and transmembrane helix, reflecting its role in neuronal signaling.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (residues 23–240), which is predicted to be exposed on the cell surface; this region may be suitable for applications detecting native receptor complexes.
• For Western blotting, CHRNA5 has a predicted molecular weight of approximately 53 kDa; consider using reducing and non-reducing conditions to optimize detection of native and denatured forms.
• In ELISA, the recombinant protein immunogen can serve as a positive control to validate antibody binding; cross-reactivity with other alpha subunits should be assessed if applied to complex samples.
• Due to neuronal expression, brain tissue or neuronal cell lines (e.g., SH-SY5Y) are recommended as positive sample sources; antibody specificity should be confirmed using knockout controls or blocking peptides.

CamelBio: Your One-Stop Sourcing Bridge

As a comprehensive IVD raw material supplier, CamelBio provides reliable access to this CHRNA5 antibody along with a portfolio of validated antibodies, antigen pairs, and ancillary reagents for neuroscience and addiction research. We support diagnostic manufacturers and research laboratories with one-stop sourcing of high-quality monoclonal and polyclonal antibodies, recombinant proteins, and custom services to accelerate your project from concept to clinic. Explore our range of nAChR-targeted tools to advance your studies in synaptic transmission and neurological disorders.