Anti-CHRNA3 Rabbit Polyclonal Antibody for WB and ELISA - P32297

Core Product Specifications and Parameters

Biological Background: CHRNA3 Function and Localization

• Neuronal acetylcholine receptor subunit alpha-3, a component of pentameric ligand-gated cation channels (PubMed:31488329, PubMed:31708116). Related references: PMID:31488329, PMID:31708116
• Forms heteropentamers with CHRNB2, CHRNB4, CHRNA5, and CHRNB3, with CHRNA3:CHRNB4 being predominant in autonomic ganglia (PubMed:20881005, PubMed:8663494). Related references: PMID:20881005, PMID:8663494
• Plays a role in the habenulo-interpeduncular tract, modulating the mesolimbic dopamine system and addiction (By similarity).
• Hypothalamic CHRNA3:CHRNB4 activation by nicotine regulates POMC neurons and food intake (By similarity).
• Expressed in urothelium where it modulates reflex bladder activity via calcium influx and ATP release (By similarity).
• Subcellularly located at the synaptic cell membrane, cell membrane, endoplasmic reticulum, and Golgi apparatus.
• Post-translational modifications include glycosylation, disulfide bonds, phosphorylation, and ubiquitination; also features alternative splicing.
• Associated with disease variants (keyword: Disease variant).

Experimental Guidance and Technical Tips

• The immunogen used for this antibody corresponds to amino acids 32-240 of human CHRNA3, a region that may represent an extracellular domain important for ligand binding and subunit interactions.
• For Western blot applications, consider using lysates from neuronal tissues or cell lines expressing CHRNA3, as the protein has a predicted weight of 57 kDa.
• The antibody cross-reacts with human, mouse, and rat samples, making it suitable for comparative studies; however, endogenous expression levels should be verified in the specific system under study.
• ELISA may be used for detecting CHRNA3 in purified samples or for quantitative assays; optimization of coating and blocking conditions is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For neuroscience and receptor research, we offer high-quality CHRNA3 antibodies like this polyclonal antibody, and we also excel at sourcing rare-target raw materials to accelerate your diagnostic and research workflows.