Anti-CHRM5 Polyclonal Antibody for WB and ELISA - P08912

Core Product Specifications and Parameters

Biological Background: CHRM5 Function and Localization

• CHRM5 encodes the muscarinic acetylcholine receptor M5, a member of the G protein-coupled receptor (GPCR) family that mediates cellular responses to acetylcholine.
• Upon activation, the receptor primarily couples to G proteins to stimulate phosphoinositide turnover, leading to downstream signaling cascades.
• It also mediates inhibition of adenylate cyclase and modulation of potassium channels through G protein action, contributing to diverse physiological responses.
• The receptor is localized at the cell membrane, specifically enriched in the postsynaptic membrane of neurons, where it modulates synaptic transmission.
• As a transmembrane protein, it contains seven transmembrane helices and undergoes post-translational modifications including glycosylation, phosphorylation, and disulfide bond formation.
• The protein is annotated under keywords such as receptor, transducer, synapse, and glycoprotein, reflecting its role in signal transduction at synaptic junctions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the third intracellular loop (275–370) of CHRM5, a region often used to generate subtype-specific antibodies; consider sequence alignment with other muscarinic subtypes if specificity is critical.
• For Western blot, a band around 60 kDa is expected; validate in relevant tissue or cell lysates (e.g., brain regions expressing CHRM5) and use appropriate controls.
• For ELISA, the recombinant protein immunogen can serve as a positive control to assess antibody binding; optimize coating and detection conditions for indirect ELISA formats.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers streamlined access to high-quality IVD raw materials for neuroscience and GPCR research, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and ancillary reagents in bulk. Our supply network supports muscarinic receptor-related projects with reliable polyclonal antibodies like anti-CHRM5, enabling diagnostic and research applications from discovery to development. With comprehensive technical services and consulting, CamelBio bridges the gap between concept and clinic for your IVD needs.