Anti-CHRM1 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P11229

Core Product Specifications and Parameters

Biological Background: CHRM1 Function and Localization

• Muscarinic acetylcholine receptor M1 (CHRM1), also known as M1, HM1, or M1R, is a member of the G protein-coupled receptor (GPCR) superfamily.
• It mediates diverse cellular responses by coupling to G proteins, primarily involving phosphoinositide (PI) turnover, leading to the breakdown of phosphoinositides and inhibition of adenylate cyclase.
• CHRM1 modulates potassium channel activity through G protein signaling, contributing to neuronal excitability and synaptic transmission.
• The receptor is localized at the cell membrane, specifically enriched at postsynaptic cell membranes, where it responds to the neurotransmitter acetylcholine.
• CHRM1 is subject to post-translational modifications such as glycosylation and phosphorylation, and contains disulfide bonds essential for its structural integrity.
• As a key muscarinic acetylcholine receptor, CHRM1 is involved in cognitive and motor functions, making it a target for neurological research.
• Alternative splicing of the CHRM1 gene may generate multiple isoforms, potentially diversifying its functional roles.

Experimental Guidance and Technical Tips

• For Western blot analysis, a specific band around 51 kDa is expected; validate the observed molecular weight in your specific sample system.
• Given the immunogen lies within an intracellular loop, permeabilization may be required for immunofluorescence (IF/ICC) to allow antibody access; consider optimizing permeabilization conditions.
• When using immunohistochemistry (IHC-P), antigen retrieval methods may enhance detection; pilot experiments are recommended to establish the optimal protocol.
• The antibody is suitable for ELISA applications, but working dilutions should be empirically determined for your assay format.
• Cross-reactivity with mouse and rat makes this antibody a valuable tool for studying CHRM1 in common rodent models.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing channel for IVD raw materials, connecting diagnostic manufacturers and research labs with validated polyclonal antibodies like this anti-CHRM1 pAb, along with optimized ancillary reagents. Our supply capabilities extend to bulk monoclonal/polyclonal antibodies and rare-target raw materials, supporting your GPCR signaling and neuroscience research programs efficiently from concept to clinic.