Anti-CHRM1 Polyclonal Antibody for WB, IF/ICC, ELISA - P11229

Core Product Specifications and Parameters

Biological Background: CHRM1 Function and Localization

• CHRM1 encodes the muscarinic acetylcholine receptor M1, a G protein-coupled receptor (GPCR) that primarily transduces signals via phosphoinositide (Pi) turnover.
• The receptor mediates diverse cellular responses including inhibition of adenylate cyclase, breakdown of phosphoinositides, and modulation of potassium channels through heterotrimeric G proteins.
• It localizes to the cell membrane, specifically the postsynaptic cell membrane, consistent with its role in synaptic transmission.
• Belongs to the GPCR superfamily, characterized by seven transmembrane domains, and undergoes alternative splicing to produce multiple isoforms.
• Post-translational modifications include glycosylation, phosphorylation, and disulfide bond formation, which are important for receptor function and stability.
• The 3D structure has been determined, offering valuable insights into ligand binding and activation mechanisms.

Experimental Guidance and Technical Tips

• The immunogen comprises a synthetic peptide from the N-terminal region (aa 1-100) of human CHRM1; for western blot, expect a band near 51 kDa and validate expression in relevant cell or tissue lysates from human, mouse, or rat.
• For immunofluorescence/immunocytochemistry, optimize permeabilization and fixation conditions, and include appropriate negative controls to confirm specificity.
• In ELISA applications, consider using the antibody for indirect detection; titrate carefully and include antigen-coated and antibody-only controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research institutions with comprehensive IVD raw material solutions, from validated antibody pairs and bulk reagents to rare-target sourcing. The CHRM1 polyclonal antibody exemplifies our supply capabilities for neuroscience and GPCR research, enabling reliable assay development. Explore our integrated procurement services to accelerate your diagnostic projects from concept to clinic.