Anti-CHRFAM7A Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q494W8

Core Product Specifications and Parameters

Biological Background: CHRFAM7A Function and Localization

• CHRFAM7A encodes a CHRNA7-FAM7A fusion protein, also known as CHRNA7-DR1 or D-10, resulting from a human-specific partial gene duplication and fusion event.
• The protein contains the N-terminal region of the alpha-7 nicotinic acetylcholine receptor (CHRNA7) fused to the FAM7A (family with sequence similarity 7 member A) protein.
• Subcellular localization is predicted to be the membrane, consistent with the presence of transmembrane domains inherited from the CHRNA7 subunit.
• Tissue specificity studies indicate expression in the hippocampus, suggesting a potential role in neuronal signaling or synaptic function.
• The fusion protein is classified as a reference proteome and is annotated with transmembrane helix motifs, underscoring its integration into lipid bilayers.
• As a chimeric gene product, CHRFAM7A may contribute to human-specific neurobiological adaptations and is a candidate for studying genomic duplication events.
• The protein lacks known ligand-gated ion channel activity due to the truncation and replacement of key functional domains from CHRNA7 by FAM7A sequences.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1-150 of human CHRFAM7A, targeting the N-terminal region shared with CHRNA7. Pre-adsorption controls may help distinguish CHRFAM7A-specific signals from cross-reactivity with full-length CHRNA7.
• For Western blot (WB), the predicted molecular weight is 46 kDa; consider using brain tissue lysates (e.g., hippocampus) as a positive control, and validate with siRNA or knockout models if available.
• Immunohistochemistry (IHC-P) on paraffin-embedded sections may require antigen retrieval; staining in human hippocampal sections should be verified with appropriate negative controls.
• In ELISA, the antibody can be used as capture or detection reagent; confirm specificity using recombinant CHRFAM7A protein and assess cross-reactivity against CHRNA7.
• When working with mouse or rat samples, verify reactivity in the target tissue due to potential species-specific expression patterns.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive one-stop platform for IVD raw-material sourcing, including this anti-CHRFAM7A antibody, tailored for diagnostic manufacturers, laboratories, and research institutes. Our portfolio encompasses validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized sourcing for rare targets like the CHRNA7-FAM7A fusion protein. We support your neuroscience and receptor biology projects from concept to clinic with reliable supply and technical consulting.