Anti-CHRFAM7A Rabbit Polyclonal Antibody for WB, ELISA - Q494W8

Core Product Specifications and Parameters

Biological Background: CHRFAM7A Function and Localization

• CHRFAM7A is a human-specific fusion protein produced by the combination of CHRNA7 and FAM7A gene sequences.
• It is also known as D-10, CHRNA7-DR1, or CHRNA7-FAM7A fusion protein, encoded by the CHRFAM7A gene.
• The protein is predicted to be a membrane protein containing transmembrane helices, consistent with its association with the nicotinic acetylcholine receptor family.
• Subcellular localization is to the membrane, as annotated in UniProt.
• It is predominantly expressed in the hippocampus, suggesting a role in neuronal function.
• This entry is part of the UniProt reference proteome, indicating its importance as a well-characterized human protein.

Experimental Guidance and Technical Tips

• The polyclonal antibody was generated in rabbit against the N-terminal region (amino acids 1-150) of human CHRFAM7A. For Western blotting, consider the predicted molecular weight of approximately 46 kDa when selecting gel percentage and transfer conditions.
• In ELISA applications, coating with the immunogen or a peptide derived from the target region may be used to assess antibody reactivity. Serial dilutions should be tested to determine optimal working concentrations.
• Cross-reactivity with mouse and rat is reported, but validation in the specific sample system and application is recommended. Include appropriate positive and negative controls, especially when analyzing tissues other than hippocampus.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for neurobiology targets, offering polyclonal and monoclonal antibodies validated for core applications. For CHRFAM7A-focused research, we supply high-quality antibody pairs, bulk ancillary reagents, and customized sourcing of rare targets to support your assay development workflow with reliable, single-vendor convenience.