Anti-CHRD Rabbit Polyclonal Antibody for WB, ELISA - Q9H2X0

Core Product Specifications and Parameters

Biological Background: CHRD Function and Localization

• CHRD encodes Chordin, a secreted key developmental protein that acts as a dorsalizing factor by binding to and sequestering BMPs (bone morphogenetic proteins), thereby antagonizing ventralizing signals during early embryogenesis.
• The protein contains von Willebrand factor type C (VWC) repeats that are critical for BMP binding and inhibition.
• Alternative splicing produces multiple isoforms of CHRD, which may exhibit tissue- or stage-specific expression and functional divergence.
• Subcellular localization is exclusively secreted, consistent with its role in modulating extracellular morphogen gradients.
• Post-translational modifications include glycosylation, which can affect protein stability and activity.
• CHRD is expressed at the highest level in the adult liver, though its embryonic expression is more widespread, particularly in the node and notochord.
• As a member of the chordin/chordin-like family, it contributes to the fine-tuning of TGF-β superfamily signaling, with potential implications in organogenesis and tissue homeostasis.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (aa 686–955) of human CHRD; consider that epitope recognition may be influenced by protein processing or interactions with binding partners.
• For Western blotting, anticipate a band near the predicted molecular weight of 102 kDa; inclusion of a positive control lysate from liver or cells overexpressing CHRD is advisable.
• Given the polyclonal nature, lot-to-lot variability may occur; assess specificity using blocking peptides or knockout tissue when available.
• Cross-reactivity with mouse and rat has been confirmed by the supplier; perform an initial validation in your specific experimental system to ensure signal fidelity.
• In ELISA applications, this antibody can be employed as a capture or detection reagent following standard titration and optimization under your assay conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement by offering validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents for targets like CHRD, a crucial developmental protein. Our supply network covers rare-target sourcing and supports your transition from concept to clinic, ensuring ready access to high-quality immunoreagents for immunoassay development and research applications.