Anti-CHRAC1 Rabbit Polyclonal Antibody for WB, ELISA - Q9NRG0

Core Product Specifications and Parameters

Biological Background: CHRAC1 Function and Localization

• CHRAC1, also known as chromatin accessibility complex 15 kDa protein (CHRAC-15) and DNA polymerase epsilon subunit p15, is a small protein of approximately 14.7 kDa encoded by the CHRAC1 gene (Gene ID 54108).
• Forms a complex with DNA polymerase epsilon subunit POLE3 and binds naked DNA, which is then incorporated into chromatin, aided by the nucleosome remodeling activity of ISWI/SNF2H and ACF1. Does not enhance nucleosome sliding activity. Related references: PMID:14759371
• Predominantly localized to the nucleus, consistent with its role in chromatin dynamics.
• Widely expressed across human tissues, including heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas.
• Annotated with post-translational modifications such as acetylation and phosphorylation, suggesting regulatory mechanisms.
• Classified under keywords including DNA-binding, coiled-coil, nucleotidyltransferase, and phosphoprotein, highlighting its functional versatility.

Experimental Guidance and Technical Tips

• For Western blot, consider starting with a dilution range of 1:500–1:2000 and optimize based on your specific sample type and detection system.
• In ELISA, use the recombinant immunogen or purified CHRAC1 protein for coating to determine optimal antibody titration.
• The antibody was raised against an N-terminal fragment (aa 1–131) and may detect both full-length and processed forms; validate in human cell lines or tissue lysates relevant to your experimental context.
• Cross-reactivity is confirmed for human; if testing other species, perform sequence alignment and preliminary assays to assess reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive access to IVD raw materials, technical services, and consulting, supporting every stage from concept to clinic. For chromatin and DNA replication research, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies like this CHRAC1 pAb, bulk ancillary reagents, and rare target sourcing. Partner with us to streamline your assay development and procurement needs.