Anti-CHRAC1 Polyclonal Antibody for WB, ELISA - Q9NRG0

Core Product Specifications and Parameters

Biological Background: CHRAC1 Function and Localization

• CHRAC1, also known as CHRAC-15, CHARC1, YCL1, and C1, is a 15 kDa chromatin accessibility complex protein 1, a subunit of the DNA polymerase epsilon complex.
• Forms a complex with DNA polymerase epsilon subunit POLE3 and binds naked DNA, facilitating its incorporation into chromatin. This process is aided by the nucleosome remodeling activity of ISWI/SNF2H and ACF1.
  Related references: PMID:14759371
• Does not enhance the nucleosome sliding activity of the ACF-5 ISWI chromatin remodeling complex, indicating a specific role in chromatin assembly rather than active remodeling.
• Predominantly localized in the nucleus, consistent with its function in chromatin dynamics.
• Widely expressed across multiple human tissues, including heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas.
• Post-translational modifications include acetylation and phosphorylation, suggesting potential regulatory mechanisms.
• Contains a coiled-coil domain and is involved in DNA-binding, linking chromatin remodeling and DNA replication.

Experimental Guidance and Technical Tips

• For Western blot, use a high-percentage SDS-PAGE gel (e.g., 15%) to effectively resolve the small ~15 kDa CHRAC1 protein.
• The antibody has been knockout validated, which indicates target specificity; include appropriate negative controls (e.g., knockout cell lysate) in your experiments.
• Due to its polyclonal nature, minor additional bands may appear; confirm specificity with an independent method such as siRNA knockdown or overexpression.
• For ELISA, the recombinant immunogen (amino acids 1-131 of human CHRAC1) can be used as a coating antigen to determine antibody titer and specificity.
• Cross-reactivity with mouse and rat is reported; validate performance in your species of interest and consider sequence alignment to ensure epitope conservation.

CamelBio: Your One-Stop Sourcing Bridge

As a dedicated partner for diagnostic manufacturers and research laboratories, CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents. This anti-CHRAC1 polyclonal antibody supports chromatin and epigenetics research applications, and we can assist with sourcing rare target reagents or custom development to meet your specific assay requirements.