Anti-CHPT1 Polyclonal Antibody for WB, ELISA - Q8WUD6

Core Product Specifications and Parameters

Biological Background: CHPT1 Function and Localization

• CHPT1 (also known as CPT1 or CPT) is a cholinephosphotransferase that catalyzes the final step of de novo phosphatidylcholine (PC) synthesis, transferring choline phosphate from CDP-choline to diacylglycerol (DAG).
• The protein is integral to membrane biogenesis, playing a central role in the formation and maintenance of vesicular membranes.
• CHPT1 localizes to the Golgi apparatus membrane.
• It is highly expressed in testis, colon, small intestine, heart, prostate, and spleen; detected at lower levels in several other tissues; and is overexpressed in cancerous breast epithelial cell lines.
• The enzyme requires magnesium or manganese as cofactors and contains at least one transmembrane helix.
• The CHPT1 gene undergoes alternative splicing, and the protein is subject to acetylation.
• A 3D structure of the protein has been solved, providing insights into its enzymatic mechanism.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to the N-terminal region (amino acids 1-100) of human CHPT1. This antibody may recognize full-length CHPT1 in denaturing western blot; validate in your sample system.
• For ELISA applications, consider using the immunogen peptide as a positive control to assess antibody binding efficiency.
• Optimize antibody dilution for WB and ELISA based on your experimental conditions. If recommended dilutions are not available, perform a titration curve.
• When preparing lysates, include appropriate detergents to solubilize membrane proteins, as CHPT1 is a Golgi membrane protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers streamlined access to IVD raw materials, technical services, and consulting. For lipid metabolism and cancer-related targets like CHPT1, we provide validated polyclonal antibodies, optimized antibody pairs, and bulk ancillary reagents to support your assay development. If you need customized sourcing for rare targets or large-scale production, contact our team to discuss your requirements.