Anti-CHP1 Polyclonal Antibody for WB, IF/ICC, ELISA - Q99653

Core Product Specifications and Parameters

Biological Background: CHP1 Function and Localization

• CHP1 (calcineurin B homologous protein 1) is a 22-kDa EF-hand calcium-binding protein also known as p22, SLC9A1BP; it undergoes N-myristoylation and phosphorylation.
• Mediates microtubule-membrane organelle linkage, required for transcytotic vesicle targeting and fusion with the plasma membrane.
• Integral cofactor for Na+/H+ exchanger SLC9A1/NHE1, enhancing its pH sensitivity, stabilizing its plasma membrane localization, and inhibiting serum/GTPase-stimulated exchange.
• Represses ribosomal RNA transcription by inhibiting UBF1 nucleolar translocation and binding to ribosomal gene promoters.
• Negative regulator of calcineurin/NFAT signaling, suppressing NFAT nuclear import and transcriptional activity.
• Localizes to nucleus, cytoplasm, cytoskeleton, ER, Golgi, and plasma membrane; ubiquitously expressed with detection in multiple fetal tissues.

Experimental Guidance and Technical Tips

• For Western blot, the expected band is ~22 kDa; consider using whole-cell lysate from human, mouse, or rat cell lines.
• For IF/ICC, fixation/permeabilization conditions may need optimization given multi-organelle localization; validate in cells expressing CHP1 (e.g., HeLa, HEK293).
• ELISA performance: the immunogen is a recombinant protein; ensure coating conditions and detection system are compatible with rabbit polyclonal antibodies.
• Cross-reactivity with mouse and rat has been reported; verify sequence conservation if using non-human samples.
• As a polyclonal antibody, lot-to-lot variability may occur; consider validating new lots for critical applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive sourcing service for IVD raw materials, from concept to clinic. For CHP1-related research—spanning pH regulation, vesicle trafficking, and calcineurin signaling—we supply validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and can assist with rare target raw-material procurement. Our expertise ensures reliable access to critical reagents for diagnostic development.