Anti-CHODL Rabbit Polyclonal Antibody for WB, ELISA - Q9H9P2

Core Product Specifications and Parameters

Biological Background: Chondrolectin Function and Localization

• Chondrolectin (CHODL) is a transmembrane protein also known as MT75, encoded by the CHODL gene (C21orf68).
• It may function in nervous system development, potentially promoting neurite outgrowth and elongation, as well as motor axon growth and guidance.
• CHODL exhibits alternative promoter usage and alternative splicing, generating multiple isoforms; isoform 2 is predominantly expressed in B lymphocytes and mature thymocytes, while isoform 3 is specifically expressed in peripheral blood T lymphocytes.
• The protein localizes to the cytoplasm, membranes, and endoplasmic reticulum (ER) membrane.
• CHODL is a lectin family member containing a signal peptide, a transmembrane helix, and disulfide bonds; it is classified as a glycoprotein.
• Tissue expression is observed in spleen, testis, prostate, fetal liver, heart, skeletal muscle, and small intestine, with isoform-specific patterns in immune cells.

Experimental Guidance and Technical Tips

• The immunogen consists of a recombinant fusion protein spanning amino acids 110–210 of human CHODL. As a polyclonal antibody, it is expected to recognize multiple epitopes within this region.
• For Western blot (WB), a band around 30 kDa (consistent with the predicted molecular weight of 30–31 kDa) may be observed. Due to alternative splicing, additional isoforms may appear; validate in appropriate tissue or cell lysates.
• Given the cross-reactivity with mouse and rat, the antibody may be suitable for detecting orthologs in these species; however, sequence homology in the immunogen region should be checked.
• For ELISA, the recombinant protein fragment (or a peptide from the same region) could be used as a coating antigen. Titration and optimization are recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, supplying high-quality antibodies such as this CHODL polyclonal antibody for studying chondrolectin in nervous system development. Our capabilities encompass validated antibody pairs, optimized mono- and polyclonal antibodies, bulk ancillary reagents, and bespoke sourcing for rare targets—all designed to accelerate your assay development.