Anti-CHN1 Rabbit Polyclonal Antibody for WB and ELISA - P15882

Core Product Specifications and Parameters

Biological Background: CHN1 Function and Localization

• CHN1 encodes N-chimaerin, also known as A-chimaerin, alpha-chimerin, and Rho GTPase-activating protein 2.
• It functions as a GTPase-activating protein (GAP) for p21-rac and acts as a phorbol ester receptor.
• CHN1 is critical for neuronal development, participating in locomotor circuit assembly and axon guidance as a direct effector of EPHA4.
• In humans, CHN1 is primarily expressed in brain regions involved in learning and memory.
• The protein features an SH2 domain and a zinc-finger domain, and is regulated by phosphorylation and acetylation.

Experimental Guidance and Technical Tips

• The immunogen covers the N-terminal region (aa 1-200), which may include part of the SH2 domain; thus, the antibody could potentially recognize full-length and some truncated CHN1 forms. Validate specificity in the relevant sample system.
• For Western blotting, a band near 53 kDa is expected. Use neuronal tissue or cell line lysates where CHN1 is enriched. Consider optimizing protein extraction and loading controls.
• In ELISA applications, titrate the antibody to determine the optimal working concentration for your specific assay format, and include appropriate negative controls.
• When using cross-reactive species (Mouse, Rat), confirm reactivity with homologous target sequences in pilot experiments.

CamelBio: Your One-Stop Sourcing Bridge

As a comprehensive IVD raw-material partner, CamelBio streamlines access to a wide array of validated antibodies, bulk ancillary reagents, and custom production services, supporting every stage from concept to clinical application. This anti-CHN1 polyclonal antibody is well-suited for neuroscience-focused assay systems, and CamelBio’s global sourcing network can assist in obtaining rare-target raw materials or scaling up to meet your diagnostic development needs.