Anti-CHMP6 Polyclonal Antibody for WB, IF/ICC, ELISA - Q96FZ7

Core Product Specifications and Parameters

Biological Background: CHMP6 Function and Localization

• CHMP6 (charged multivesicular body protein 6), also designated Vps20 or chromatin-modifying protein 6, is a probable core component of the endosomal sorting required for transport complex III (ESCRT-III).
• It is essential for multivesicular body (MVB) formation, mediating the sorting of endosomal cargo proteins into intraluminal vesicles (ILVs) that are destined for lysosomal degradation.
• The ESCRT-III machinery, including CHMP6, participates in topologically equivalent membrane fission events such as the terminal stages of cytokinesis and the budding of enveloped viruses (e.g., HIV-1).
• Within the ESCRT-III complex, CHMP6 likely functions as an acceptor for the ESCRT-II complex on endosomal membranes, facilitating the recruitment of downstream ESCRT-III subunits.
• Subcellular localization includes the endomembrane system, endosome membrane, and late endosome membrane, consistent with its role in endosomal trafficking.
• CHMP6 is ubiquitously expressed across human tissues, indicating a fundamental housekeeping function in cellular membrane dynamics.
• Post‑translational modifications include myristoylation and phosphorylation, and the protein contains coiled-coil domains critical for ESCRT-III filament assembly.
• Predicted molecular weight is approximately 23.5 kDa (23485 Da), consistent with the observed 23 kDa band in denaturing gels.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 111–200 of human CHMP6, a central region that may detect full‑length protein and potential isoforms; include appropriate controls in your experimental design.
• For Western blot (WB), consider using reducing conditions and validate with lysates from human or mouse cell lines expressing endogenous CHMP6.
• In immunofluorescence/immunocytochemistry (IF/ICC), optimize fixation and permeabilization protocols, as membrane‑associated proteins may require specific conditions for accurate localization.
• For ELISA, test serial dilutions of recombinant CHMP6 or cellular extracts to determine optimal antibody working concentrations and detection limits.
• Cross‑reactivity with human and mouse has been confirmed; if using other species, perform sequence alignment and, if necessary, validate reactivity experimentally.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio provides a comprehensive one‑stop sourcing solution for IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare‑target procurement. This CHMP6 antibody supports research into endosomal trafficking, ESCRT biology, and related disease mechanisms. Contact CamelBio for customized technical consulting and reliable access to the raw materials you need from concept to clinic.