Anti-CHMP4B Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9H444

Core Product Specifications and Parameters

Biological Background: CHMP4B Function and Localization

• CHMP4B is a core component of the ESCRT-III complex, essential for forming multivesicular bodies (MVBs) and sorting endosomal cargo into intraluminal vesicles, which are ultimately delivered to lysosomes for degradation (PubMed:12860994, PubMed:18209100). Related references: PMID:12860994 PMID:18209100
• It participates in topologically equivalent membrane fission events, including the terminal stages of cytokinesis (PubMed:21310966) and nuclear envelope sealing during late anaphase in conjunction with SPAST. Related references: PMID:21310966 PMID:26040712
• When overexpressed, CHMP4B forms membrane-assembled circular filaments that promote negative curvature and outward budding, facilitating exosomal release of SDCBP, CD63, and syndecan. Related references: PMID:22660413
• The majority of the protein exists in a folded closed conformation, which may regulate its activity. Related references: PMID:33349255
• CHMP4B is involved in the budding of enveloped viruses such as HIV-1, interacting with PDCD6IP/Alix to mediate virus release.
• It is widely expressed, with higher levels in heart and skeletal muscle, and also present in brain, colon, thymus, spleen, kidney, liver, small intestine, placenta, lung, and peripheral blood lymphocytes.
• Subcellular localization includes cytoplasm, cytosol, late endosome membrane, midbody, and nuclear envelope.
• Post-translational modifications include acetylation and phosphorylation; mutations in CHMP4B have been associated with cataract.

Experimental Guidance and Technical Tips

• The immunogen covers the full-length human CHMP4B sequence (aa 1-224), suggesting potential recognition of both native and denatured forms; consider optimizing denaturing conditions for Western blot with relevant controls.
• For immunofluorescence (IF/ICC), fixation and permeabilization protocols should be validated for target cell types, with expected staining patterns at endosomal and nuclear envelope structures.
• This antibody demonstrates reactivity with human, mouse, and rat samples; cross-reactivity with other species should be independently verified if required.
• ELISA applications may benefit from using the recombinant immunogen as a positive control to assess binding specificity and sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For CHMP4B-targeted research and assay development, we offer validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. Our team also supports rare target raw-material sourcing to accelerate your projects in cell biology and virology.