Anti-CHMP2B Polyclonal Antibody for WB, IF/ICC, ELISA - Q9UQN3

Core Product Specifications and Parameters

Biological Background: CHMP2B Function and Localization

• CHMP2B, also known as CHMP2.5, chromatin-modifying protein 2b, or Vps2-2, is a human protein encoded by the CHMP2B gene. It is a member of the charged multivesicular body protein family and functions as a core component of the endosomal sorting complex required for transport III (ESCRT-III).
• As part of the ESCRT-III machinery, CHMP2B plays a central role in the formation of multivesicular bodies (MVBs) and the sorting of ubiquitinated cargo into intraluminal vesicles (ILVs) within late endosomes. This process directs membrane proteins, such as activated growth factor receptors, toward lysosomal degradation.
• The ESCRT-III complex mediates membrane scission events not only in MVB biogenesis but also in topologically equivalent processes, including the final abscission step of cytokinesis and the budding of enveloped viruses like HIV-1.
• CHMP2B operates in the cytosol and is recruited to the cytoplasmic face of late endosomal membranes, where it assembles into dynamic polymers that drive membrane invagination and fission in conjunction with the AAA ATPase VPS4.
• Post-translational modifications of CHMP2B include acetylation and phosphorylation, which may regulate its assembly, localization, and function within the ESCRT pathway.
• Keywords from UniProt highlight its involvement in protein transport, endosome biology, and its association with neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and frontotemporal dementia.
• The protein is widely expressed across human tissues, with notable presence in brain regions (cerebellum, cerebral cortex, medulla, spinal cord, etc.), heart, skeletal muscle, spleen, kidney, liver, and lung, reflecting its fundamental role in cellular membrane dynamics.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody is a recombinant fusion protein corresponding to amino acids 1–213 of human CHMP2B (NP_054762.2). This sequence covers nearly the full-length protein (excluding only a short C-terminal region), which suggests the antibody may recognize multiple epitopes.
• Given its polyclonal nature and design, this antibody is suitable for Western blot (WB) analysis of endogenous CHMP2B in human and mouse samples; a band near 24 kDa is expected. Consider including appropriate positive and negative controls to confirm specificity.
• For immunofluorescence (IF) or immunocytochemistry (ICC), CHMP2B localizes to the cytoplasm with enrichment on late endosomal membranes. Permeabilized cell preparations may be necessary to detect this membrane-associated pool. Validate staining in the relevant cell system and consider co-staining with endosomal markers.
• In ELISA applications, the antibody can be used for quantitative detection, but optimal working dilutions must be determined empirically in the user’s assay system.
• Cross-reactivity with mouse has been reported, making this antibody a candidate for translational studies using mouse models. However, users should verify reactivity with their specific experimental tissues or cell lines.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research laboratories, and institutes with streamlined access to high-quality IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this anti-CHMP2B reagent, and a broad portfolio of ancillary reagents. Whether your projects involve neurodegenerative disease biomarkers, endosomal trafficking research, or rare target sourcing, we can coordinate bulk supply and technical consultation from concept to clinic. Reach out to explore how we can support your next assay development with reliable raw material solutions.