Anti-CHMP1B Rabbit Polyclonal Antibody for WB, ELISA - Q7LBR1

Core Product Specifications and Parameters

Biological Background: CHMP1B Function and Localization

• CHMP1B (Charged multivesicular body protein 1b), also known as CHMP1.5, Vps46-2, or hVps46-2, is a member of the ESCRT-III complex family involved in membrane remodeling and scission.
• It functions as a peripheral component of the ESCRT-III complex, essential for sorting ubiquitinated cargo into intraluminal vesicles of multivesicular bodies (MVBs), leading to lysosomal degradation.
• CHMP1B participates in the final stages of cytokinesis by recruiting VPS4A/B and SPAST to the midbody, facilitating abscission.
• This protein is required for the budding and release of enveloped viruses, such as HIV-1, through interactions with viral p6 and p9 motifs.
• Subcellularly, CHMP1B localizes to the cytosol, late endosome membrane, and endosomes, consistent with its role in endosomal trafficking.
• It is widely expressed, with detected levels in pancreas, kidney, skeletal muscle, liver, lung, placenta, and brain.
• Structurally, CHMP1B contains coiled-coil domains typical of ESCRT-III subunits and has a molecular weight of approximately 22 kDa.
• Post-translational modifications and membrane association regulate its polymerization and interaction with AAA ATPases like VPS4 for membrane fission.

Experimental Guidance and Technical Tips

• The immunogen used for this antibody is a recombinant fusion protein encompassing amino acids 1-199 of human CHMP1B. For experiments involving truncated or mutated forms, verify that the epitope is within this region.
• While validated for Western blot (WB) and ELISA, consider optimizing dilutions for your specific tissue and cell lysates; the predicted molecular weight is 22 kDa, but band shifts may occur due to post-translational modifications.
• Given cross-reactivity with human, mouse, and rat, this antibody is suitable for comparative studies across these species; however, primary sequence alignment may help predict reactivity with other organisms.
• For ELISA, using the recombinant immunogen as a positive control can assist in assay development and titration.

CamelBio: Your One-Stop Sourcing Bridge

With expertise in ESCRT biology and cell division research, CamelBio offers CHMP1B antibodies and related IVD raw materials, including validated antibody pairs, bulk polyclonal/monoclonal antibodies, and ancillary reagents. From early-stage assay development to clinical manufacturing, we help diagnostic manufacturers and labs secure high-quality reagents, streamline procurement, and address rare-target sourcing needs.