Anti-CHMP1A Polyclonal Antibody for WB and ELISA - Q9HD42

Core Product Specifications and Parameters

Biological Background: CHMP1A Function and Localization

• CHMP1A (charged multivesicular body protein 1a), also known as chromatin-modifying protein 1a, is a peripheral component of the endosomal sorting complex required for transport III (ESCRT-III).
• It plays a central role in multivesicular body (MVB) formation and sorting of endosomal cargo proteins into intraluminal vesicles (ILVs) for subsequent lysosomal degradation.
• CHMP1A participates in topologically equivalent membrane fission events, including the terminal stages of cytokinesis and the budding of enveloped viruses such as HIV-1.
• It recruits the AAA ATPase VPS4A and/or VPS4B to the midbody of dividing cells and may also be involved in chromosome condensation.
• CHMP1A targets the Polycomb group protein BMI1/PCGF4 to regions of condensed chromatin, thereby contributing to PcG gene silencing and stable cell cycle progression.
• The protein localizes to the cytoplasm, endosome membrane, and nuclear matrix, and is expressed in placenta, skin fibroblasts, and osteoblasts.
• Post-translational modifications include acetylation and phosphorylation; alternative splicing generates multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 1-196) covers a substantial N-terminal region; consider verifying reactivity against endogenously expressed full-length CHMP1A in relevant cell or tissue lysates.
• In Western blot, a specific band near 22 kDa is expected; use appropriate molecular weight markers and loading controls to confirm specificity.
• For ELISA applications, test direct and indirect formats with optimized antigen coating and detection conditions; cross-reactivity with mouse CHMP1A may require separate validation.
• Because CHMP1A functions in cell division, proliferating or synchronized cell populations may yield stronger signals; include appropriate positive and negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes streamlined access to IVD raw materials, including antibodies for ESCRT biology and chromatin regulation. Beyond this anti-CHMP1A polyclonal antibody, we can supply validated antibody pairs, optimized monoclonal reagents, and bulk ancillary materials. For rare or specialized targets such as CHMP1A, our global sourcing network helps secure the raw materials needed to advance your projects from concept to clinic.