Anti-CHML Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P26374

Core Product Specifications and Parameters

Biological Background: CHML Function and Localization

• CHML, also known as Rab escort protein 2 (REP-2) or choroideremia-like protein, acts as the substrate-binding subunit (component A) of the Rab geranylgeranyltransferase (GGTase) complex.
• It binds unprenylated Rab proteins and presents them to the catalytic component B for geranylgeranylation, which is essential for proper Rab membrane association and intracellular trafficking.
• CHML is less effective than CHM (REP-1) in supporting prenylation of the Rab3 family, indicating distinct substrate specificities within the Rab escort protein family.
• Subcellularly, CHML localizes to the cytoplasm and cytosol, where it participates in the Rab prenylation and recycling cycle.
• The protein is subject to phosphorylation (Keywords: Phosphoprotein) and is associated with GTPase activation, potentially modulating Rab-mediated signaling pathways.

Experimental Guidance and Technical Tips

• The immunogen spans residues 1–100 at the N-terminus of human CHML; consider that epitope accessibility may vary depending on sample preparation and assay conditions.
• For Western blot, a band around 74 kDa is expected; validate with positive controls from human, mouse, or rat tissues/cells based on the reported cross-reactivity.
• In immunofluorescence/ICC, detection of cytoplasmic CHML may require optimized fixation and permeabilization steps.
• ELISA performance may depend on coating with native or recombinant CHML protein; appropriate blocking strategies should be evaluated.
• When using mouse or rat models, confirm antibody specificity by parallel testing with human samples or recombinant proteins.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a streamlined channel for sourcing IVD raw materials, including validated antibodies such as this anti-CHML polyclonal antibody for Rab prenylation research. Our supply capabilities extend to optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets, supporting your entire development workflow from concept to clinic.