Anti-Chk2 Rabbit Polyclonal Antibody for WB, IF/ICC, IP, ELISA - O96017

Core Product Specifications and Parameters

Biological Background: Chk2 Function and Localization

• Chk2 (Checkpoint kinase 2, encoded by CHEK2) is a serine/threonine-protein kinase that acts as a central mediator of the DNA damage response, phosphorylating downstream effectors to enforce cell cycle arrest, DNA repair, or apoptosis upon double-strand breaks.
• It preferentially phosphorylates substrates at the consensus sequence [L-X-R-X-X-S/T] (PubMed:37943659). Related references: PMID:37943659
• Chk2 regulates the G1/S, S, and G2/M checkpoints by phosphorylating and inhibiting CDC25 phosphatases (CDC25A, B, C), thereby maintaining inhibitory tyrosine phosphorylation on CDK-cyclin complexes.
• It promotes DNA repair through homologous recombination by phosphorylating BRCA2, which enhances RAD51 loading onto chromatin, and stimulates repair gene transcription via FOXM1 activation.
• As a tumor suppressor, Chk2 also triggers apoptosis by phosphorylating p53/TP53 at Ser-20, reducing MDM2-mediated degradation, and by activating the transcription factor E2F1.
• Chk2 mediates CCAR2-dependent inhibition of SIRT1, a process critical for p53 acetylation and apoptosis (PubMed:25361978). Related references: PMID:25361978
• Under oxidative stress, Chk2 phosphorylates TRIM32 at Ser-55, promoting ATG7 ubiquitination and positively regulating autophagy (PubMed:37943659). Related references: PMID:37943659
• Chk2 is predominantly localized in the nucleus, including PML bodies and the nucleoplasm, and is highly expressed in testis, spleen, colon, and peripheral blood leukocytes.

Experimental Guidance and Technical Tips

• The immunogen encompasses the N-terminal region (residues 1-220) of human Chk2, which includes the kinase domain; consider epitope accessibility under different fixation and denaturing conditions.
• For Western blotting, the predicted molecular weight is ~61 kDa, but additional bands may appear due to alternative splicing or post‑translational modifications; validate specificity using appropriate controls.
• This polyclonal antibody is applicable for immunoprecipitation and ELISA; for IF/ICC, optimization of permeabilization and blocking steps is recommended to minimize background.
• Since Chk2 activation is stimulated by DNA damage, treatment of cells with genotoxic agents (e.g., etoposide, ionizing radiation) may enhance detection of phosphorylated forms.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to premium IVD raw materials, including validated primary antibodies, matched pairs, and ancillary reagents. The anti-Chk2 polyclonal antibody supports investigations into DNA damage signaling, cell cycle control, and cancer biology, and can be complemented by our extensive portfolio of apoptosis and checkpoint pathway reagents. Our sourcing specialists assist from concept to clinic, ensuring reliable supply of bulk materials and custom-sourced rare targets for assay development.