Anti-Chk1 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O14757

Core Product Specifications and Parameters

Biological Background: Chk1 Function and Localization

• Chk1 (Checkpoint kinase-1) is a serine/threonine-protein kinase encoded by the CHEK1 gene, functioning as a key cell cycle checkpoint kinase.
• Required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. Related references: PMID:11535615, PMID:12399544, PMID:12446774
• Recognizes the substrate consensus sequence [R-X-X-S/T] and phosphorylates CDC25A, CDC25B, and CDC25C, leading to their inhibition via 14-3-3 binding or proteasomal degradation, thereby blocking cell cycle progression. Related references: PMID:9278511, PMID:12676583, PMID:12676925, PMID:12759351, PMID:19734889, PMID:20090422
• Phosphorylates RAD51 at Thr-309, enhancing its chromatin association to promote DNA repair by homologous recombination. Related references: PMID:15665856
• Phosphorylates TP53 on multiple C-terminal sites, promoting TP53 activation by acetylation, leading to cell cycle arrest and suppression of proliferation. Related references: PMID:10673501, PMID:15659650, PMID:16511572
• Modulates chromatin assembly by phosphorylating TLK1, preventing ASF1A phosphorylation, and participates in DNA crosslink repair via FANCE phosphorylation. Related references: PMID:12660173, PMID:12955071, PMID:17296736
• Localizes to the nucleus, chromosome, cytoplasm, and centrosome.
• Ubiquitously expressed, with highest levels in thymus, testis, small intestine, and colon.

Experimental Guidance and Technical Tips

• The immunogen spans a conserved central region (aa 148–307) of Chk1, supporting cross-reactivity with human, mouse, and rat. Validation in the relevant species is recommended.
• For Western blotting, a band around 54 kDa is expected. Thymus or testis lysates, which express high Chk1 levels, may serve as positive controls.
• When performing IHC-P or IF/ICC, consider optimization of fixation and permeabilization to enhance detection of nuclear and centrosomal Chk1.
• ELISA can be used for quantifying Chk1 in cell lysates or recombinant protein preparations; calibration against a standard curve in the specific matrix is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop partner for IVD raw materials, offering a comprehensive range of validated antibodies, antibody pairs, and ancillary reagents to support cell cycle and DNA damage research. Whether you need bulk quantities of this Chk1 polyclonal antibody, optimized monoclonal alternatives, or custom sourcing for rare targets, our team ensures seamless procurement from concept to clinic.