Anti-Chicken IL12B Rabbit Polyclonal Antibody for WB and ELISA - Q6X0K9

Core Product Specifications and Parameters

Biological Background: Interleukin-12 subunit beta Function and Localization

• Interleukin-12 subunit beta is the p40 subunit of the cytokine interleukin-12 (IL-12).
• It is also known as IL-12 subunit p40 or cytotoxic lymphocyte maturation factor 40 kDa subunit.
• The protein is secreted, consistent with its role as a circulating cytokine.
• Predicted features include an immunoglobulin-like domain, glycoprotein modifications, and intramolecular disulfide bonds.
• The calculated molecular weight is 36,149 Da, corresponding to the observed ~36 kDa band.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 21-315 of chicken IL-12B, covering the mature protein after signal peptide cleavage.
• For Western blot, use chicken immune cell lysates or recombinant protein as positive controls; expect a band at approximately 36 kDa.
• ELISA applications may require optimization of antibody dilution and pairing; suitable for direct or indirect detection formats.
• Cross-reactivity is limited to chicken IL-12B; reactivity with other species has not been tested.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to critical IVD raw materials, including this anti-chicken IL-12B antibody. Our portfolio covers validated polyclonal and monoclonal antibodies, bulk auxiliary reagents, and custom sourcing for rare targets, supporting your avian immunology and cytokine research needs from development to production.