Anti-CHIA Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9BZP6

Core Product Specifications and Parameters

Biological Background: CHIA Function and Localization

• CHIA (acidic mammalian chitinase), also known as CHIT2, AMCASE, or TSA1902, is a secreted and cytoplasmic protein that belongs to the glycosyl hydrolase 18 family, capable of degrading chitin and chitotriose.
• It plays a role in the T-helper cell type 2 (Th2) immune response and contributes to IL-13-mediated inflammation, stimulating chemokine production by pulmonary epithelial cells.
• CHIA protects lung epithelial cells against apoptosis and promotes phosphorylation of AKT1; this anti-apoptotic function is inhibited by allosamidin, indicating dependence on carbohydrate binding.
• Subcellularly, CHIA is found both secreted and in the cytoplasm, consistent with its dual roles in extracellular pathogen defense and intracellular signaling.
• Tissue specificity: highly expressed in the stomach, with lower levels detected in the lung; notably, it is detected in lung epithelial cells from asthma patients.
• Post-translational modifications include disulfide bonds and alternative splicing, as indicated by UniProt keywords.
• It is implicated in defense against nematodes and fungi, linking chitin metabolism to innate immunity.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant protein covering amino acids 1-368 of human CHIA (isoform NP_001244932.1). The antibody is expected to detect full-length CHIA at a predicted molecular weight of ~52 kDa in Western blotting under reducing conditions.
• For IHC-P, consider optimizing antigen retrieval methods and antibody dilution. The antibody has been validated for IHC-P with human, mouse, and rat samples, but optimal conditions may vary by tissue type and fixation.
• For ELISA, the antibody is suitable for detecting recombinant or endogenous CHIA; consider pairing with an appropriate detection antibody and optimize coating concentrations.
• Cross-reactivity with mouse and rat allows for translational studies across species, but sequence homology should be confirmed for the target epitope if critical.
• Note: As this is a polyclonal antibody, lot-to-lot consistency should be verified for critical applications.

CamelBio: Your One-Stop Sourcing Bridge

As a premier one-stop supplier of IVD raw materials, CamelBio provides immediate access to validated immunological reagents like this CHIA antibody to support diagnostic and research programs in chitinase biology and inflammatory diseases. Our portfolio includes optimized antibody pairs, bulk monoclonal/polyclonal antibodies, and ancillary reagents for ELISA, lateral flow, and chemiluminescence platforms. For rare target sourcing or custom antibody development needs, our technical team bridges concept to clinic with reliable raw-material supply and consultation.