Anti-CHI3L2 Polyclonal Antibody for WB, IHC-P, ELISA - Q15782

Core Product Specifications and Parameters

Biological Background: CHI3L2 (Chitinase-3-like protein 2) Function and Localization

• CHI3L2, also designated chondrocyte protein 39 or YKL-39, is a member of the chitinase-like protein family.
• It functions as a glycan-binding lectin that recognizes chitooligosaccharides and other glycans but does not display chitinase enzymatic activity.
• The protein is predominantly expressed in chondrocytes and synoviocytes, with lower levels in lung and heart; expression is absent in spleen, pancreas, and liver.
• CHI3L2 is a secreted glycoprotein, consistent with its presence in extracellular matrices.
• The protein features a chitin-binding domain and undergoes alternative splicing.
• UniProt lists evidence from 3D structure determination, direct protein sequencing, and proteomics identification.

Experimental Guidance and Technical Tips

• For Western blot detection, a protein weight of approximately 44kDa is expected; consider a gel percentage appropriate for mid-range molecular weights.
• In immunohistochemistry of paraffin-embedded sections, antigen retrieval conditions should be optimized, particularly for cartilage or synovium samples where target expression is highest.
• ELISA-based assays may be suitable for quantifying secreted CHI3L2 in culture supernatants or biological fluids.
• As the immunogen spans a large portion of the protein, the antibody may recognize different isoforms or splice variants; validation in the relevant sample system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories with comprehensive one-stop access to IVD raw materials. For studies targeting chitinase-like proteins and cartilage biology, we provide validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your development pipeline. Whether you need routine supply or specialized reagents, our team is ready to bridge your project from concept to clinic.