Anti-CHERP Polyclonal Antibody for WB, ELISA - Q8IWX8

Core Product Specifications and Parameters

Biological Background: CHERP Function and Localization

• Calcium homeostasis endoplasmic reticulum protein (CHERP), also known as SR-related CTD-associated factor 6 (SCAF6) or ERPROT 213-21, is a 104 kDa protein encoded by the CHERP gene (Gene ID: 10523) in humans.
• CHERP is involved in calcium homeostasis, growth, and proliferation, playing a role in cellular calcium regulation.
• It localizes primarily to the cytoplasm, especially the perinuclear region, and is associated with the endoplasmic reticulum.
• The protein is widely expressed in multiple tissues, including brain, placenta, lung, liver, kidney, pancreas, cardiac and skeletal muscle, and in cultured hematopoietic cells.
• CHERP undergoes post-translational modifications including acetylation, phosphorylation, and ubiquitin-like conjugation, as identified by proteomics.
• Keywords include Acetylation, Isopeptide bond, Phosphoprotein, and Ubl conjugation, indicating dynamic regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a synthetic peptide within amino acids 650–750 of human CHERP. For Western blot, consider using denaturing conditions and appropriate controls to confirm specificity.
• Due to predicted molecular weight of ~104 kDa, ensure adequate gel resolution and transfer conditions for high-molecular-weight proteins.
• For ELISA, optimizing coating antigen concentration and antibody dilution may improve signal-to-noise ratio.
• Cross-reactivity is expected with human, mouse, and rat; validate in the species-specific sample system if used beyond human.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare target sourcing. For calcium homeostasis and endoplasmic reticulum research, our CHERP antibody and related reagents offer reliable solutions from concept to clinic.