Anti-Chek2 Polyclonal Antibody for WB, ELISA - Q9Z265

Core Product Specifications and Parameters

Biological Background: Chek2 Function and Localization

• Serine/threonine-protein kinase Chk2 (also known as CHK2, Cds1, Rad53) is a critical checkpoint kinase involved in the DNA damage response.
• Functions include cell cycle arrest at G1/S and G2/M via phosphorylation of CDC25 phosphatases, leading to inhibition of CDK-cyclin complexes and blocking cell cycle progression.
• Promotes DNA repair by phosphorylating BRCA2, enhancing RAD51-mediated homologous recombination; also transcriptionally activates DNA repair genes (e.g., BRCA2) via FOXM1.
• Regulates apoptosis through phosphorylation of p53/TP53, MDM4, and PML, contributing to stabilization and activation of pro-apoptotic pathways.
• Acts as a tumor suppressor; may have a DNA damage-independent role in mitotic spindle assembly by phosphorylating BRCA1.
• Subcellular localization: predominantly nuclear, found in nucleoplasm and PML bodies.
• Expressed ubiquitously, with higher protein levels in thymus, spleen, and colon.
• Keywords: apoptosis, cell cycle, DNA damage repair, kinase, phosphoprotein, tumor suppressor.

Experimental Guidance and Technical Tips

• For Western blot, the expected molecular weight is approximately 61 kDa. Optimize antibody dilution and loading controls for your specific tissue or cell line samples.
• The immunogen corresponds to the N-terminal region (aa 1-215) of human Chek2. This region may be less conserved; verify cross-reactivity with mouse and other species if needed.
• For ELISA, consider using the recombinant protein immunogen as a positive control; titration experiments are recommended to determine optimal coating and antibody concentrations.
• Since Chek2 is activated by DNA damage, consider treating samples with genotoxic agents (e.g., etoposide, ionizing radiation) to enhance detection of phosphorylated/activated forms if using this antibody for activation studies. Note: confirmation of target specificity is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw material sourcing, from concept to clinic. This anti-Chek2 antibody supports research in DNA damage response, cell cycle checkpoints, and cancer diagnostics. We offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk reagents, and assistance with rare target sourcing to accelerate your diagnostic development.