Anti-CHD3 Rabbit Polyclonal Antibody for WB, ELISA - Q12873

Core Product Specifications and Parameters

Biological Background: CHD3 Function and Localization

• CHD3 (Chromodomain-helicase-DNA-binding protein 3), also known as Mi-2alpha, is an ATP-dependent chromatin remodeler that binds and distorts nucleosomal DNA. Related references: PMID:28977666
• It acts as a core component of the histone deacetylase NuRD complex, participating in chromatin remodeling and transcriptional repression. Related references: PMID:16428440, PMID:28977666, PMID:27068747
• CHD3 is required for anchoring centrosomal pericentrin in both interphase and mitosis, ensuring proper spindle organization and centrosome integrity. Related references: PMID:17626165
• The protein contains chromodomains, helicase domains, and zinc finger motifs, classifying it as a chromatin regulator and DNA-binding ATPase.
• CHD3 localizes primarily to the nucleus, including PML bodies, but can also be found in the cytoplasm and at the centrosome.
• It is widely expressed across tissues, highlighting its fundamental role in chromatin remodeling.
• Post-translational modifications include phosphorylation, isopeptide bond formation, and ubiquitin conjugation, which may regulate its activity and interactions.
• Alternative splicing generates multiple isoforms, potentially contributing to functional diversity.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the C-terminal region (aa 1900-2000) of human CHD3. This region may be conserved in mouse and rat, consistent with reported cross-reactivity.
• For Western blot, the predicted molecular weight is 227 kDa; consider using a suitable gel percentage and transfer conditions for high-molecular-weight proteins.
• ELISA applications may include indirect ELISA; validate with appropriate positive and negative controls.
• Verify antibody specificity in your specific sample system, as performance can vary with tissue type and preparation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and researchers streamlined access to high-quality IVD raw materials, including validated antibody pairs, monoclonal/polyclonal antibodies, and ancillary reagents. This CHD3 antibody supports chromatin remodeling and NuRD complex studies, aligning with our commitment to providing rare target raw materials for epigenetic research. Contact us to source bulk quantities or custom formulations tailored to your assay development needs.