Anti-CHD2 Polyclonal Antibody for WB, ELISA - O14647

Core Product Specifications and Parameters

Biological Background: CHD2 Function and Localization

• CHD2 is an ATP-dependent chromatin remodeler that binds to gene promoters and facilitates the deposition of histone H3.3, driving chromatin remodeling and transcriptional activation (By similarity).
• It plays a critical role in myogenesis by interacting with MYOD1, promoting H3.3 incorporation at myogenic regulatory sequences to initiate muscle gene expression (By similarity).
• CHD2 belongs to the chromodomain-helicase-DNA-binding (CHD) family, characterized by tandem chromodomains and an ATPase domain; it is involved in DNA binding, nucleosome mobilization, and transcription regulation.
• The protein localizes exclusively to the nucleus, consistent with its role in chromatin dynamics and gene expression control.
• CHD2 undergoes phosphorylation and alternative splicing, contributing to its regulatory diversity and functional modulation.
• Mutations in CHD2 are linked to developmental and epileptic encephalopathy (DEE94) and other epileptic disorders, underscoring its importance in neurodevelopment.

Experimental Guidance and Technical Tips

• The immunogen targets the N-terminal 1-223 amino acids of human CHD2; this region may recognize full-length and certain alternatively spliced isoforms.
• For western blotting, use a low-percentage gel (e.g., 6-8%) to resolve the ~211 kDa protein, and optimize transfer conditions for high-molecular-weight targets.
• Validate the antibody in relevant positive controls, such as human, mouse, or rat lysates from tissues or cell lines with known CHD2 expression (e.g., skeletal muscle, HEK293).
• Due to its polyclonal nature, multiple bands may appear; include appropriate negative controls and consider comparative peptide blocking to confirm specificity.
• For ELISA, titrate the antibody and coat with recombinant CHD2 protein or peptide to establish optimal working dilutions.

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CamelBio provides integrated access to a broad portfolio of IVD raw materials, including validated antibody pairs, optimized polyclonal antibodies like this CHD2 antibody, and bulk ancillary reagents for epigenetics and chromatin biology research. Our sourcing expertise covers rare target reagents, enabling diagnostic manufacturers and laboratories to accelerate assay development from concept to clinical application. Partner with CamelBio to streamline your supply chain and focus on your core research.