Anti-CHD2 Polyclonal Antibody for WB, ELISA - O14647

Core Product Specifications and Parameters

Biological Background: CHD2 Function and Localization

• CHD2, also known as Chromo domain-containing protein 2, is an ATP-dependent chromatin remodeling factor that regulates gene expression by altering nucleosome positioning.
• It specifically binds to promoter regions of target genes and promotes deposition of the histone variant H3.3, facilitating chromatin remodeling.
• CHD2 plays a critical role in myogenesis by interacting with MYOD1 at myogenic regulatory sequences, mediating H3.3 incorporation prior to myogenic gene activation.
• The protein contains chromodomains, an ATPase/helicase domain, and a DNA-binding domain, characteristic of the CHD family of chromatin remodelers.
• Subcellular localization is predominantly nuclear, consistent with its function in chromatin dynamics and transcriptional regulation.
• Keywords indicate involvement in transcription regulation, myogenesis, and alternative splicing, with associations to disease variants linked to epilepsy.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1729-1828 of human CHD2, a region predicted to generate an epitope-specific response; consider using the immunizing peptide for blocking or specificity controls in WB to confirm target recognition.
• For Western blot, the expected protein weight is approximately 211kDa; use appropriate gel conditions and transfer protocols for high-molecular-weight proteins.
• Validate antibody reactivity in human cell lines or tissues known to express CHD2, and include positive and negative controls to confirm performance.
• For ELISA applications, optimize coating and detection conditions using recombinant CHD2 protein or peptide as standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides IVD manufacturers, research laboratories, and diagnostic developers with comprehensive access to raw materials, technical services, and consulting—from concept to clinic. This anti-CHD2 polyclonal antibody supports chromatin biology and transcriptional regulation research, and we offer customized sourcing of validated antibody pairs, bulk ancillary reagents, and hard-to-find targets to streamline your assay development pipelines.