Anti-CHCHD7 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9BUK0

Core Product Specifications and Parameters

Biological Background: CHCHD7 Function and Localization

• CHCHD7, also known as COX23, is a coiled-coil-helix-coiled-coil-helix domain-containing protein encoded by the CHCHD7 gene in humans.
• It contains characteristic twin Cx9C motifs that form disulfide bonds, a structural feature critical for its import and folding in the mitochondrial intermembrane space.
• The protein localizes to the mitochondrial intermembrane space, where it is thought to participate in the assembly or maintenance of respiratory chain complexes.
• Alternative splicing generates multiple transcript variants, potentially giving rise to protein isoforms with distinct functional properties.
• Keywords associated with CHCHD7 include chromosomal rearrangement, suggesting its involvement in genomic translocation events that may have implications in certain cancers.
• The three-dimensional structure of CHCHD7 has been solved, providing insights into its helical bundle architecture and redox-sensitive properties.

Experimental Guidance and Technical Tips

• For Western blot (WB), the recombinant immunogen covers the full-length N-terminal region (amino acids 1-97), so the antibody is expected to detect bands near 10kDa; consider using high-percentage gels (e.g., 12–15%) and optimized transfer conditions for small proteins.
• In immunofluorescence (IF/ICC), validate mitochondrial localization by co-staining with a mitochondrial marker (e.g., Mitotracker or anti-TOMM20) in relevant human, mouse, or rat cell lines.
• For ELISA applications, the polyclonal nature allows flexible pairings; test the antibody as both capture and detection reagent and consider optimizing coating concentrations and blocking strategies for low-molecular-weight targets.
• Due to potential disulfide bond sensitivity, avoid using reducing agents in sample preparation if the detection of native conformational epitopes is desired.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with streamlined access to validated IVD raw materials. Our portfolio includes high-performance polyclonal antibodies like this CHCHD7 rabbit pAb, ideal for mitochondrial research and chromosomal rearrangement studies. With a one-stop sourcing model encompassing bulk reagents, custom antibody development, and technical consultation, we support your projects from concept to clinical application.