Anti-CHCHD6 Rabbit Polyclonal Antibody for WB, ELISA - Q9BRQ6

Core Product Specifications and Parameters

Biological Background: CHCHD6 Function and Localization

• CHCHD6 (Coiled-coil-helix-coiled-coil-helix domain-containing protein 6), also designated MIC25 or MICOS complex subunit MIC25, is a coiled-coil-helix-coiled-coil-helix domain-containing protein that functions as a component of the MICOS complex.
• The MICOS complex is a large protein assembly of the mitochondrial inner membrane that is essential for maintaining crista junction architecture, inner membrane organization, and the formation of contact sites with the outer membrane.
• CHCHD6 localizes to the mitochondrial inner membrane.
• The protein is characterized by a coiled-coil domain and is subject to post-translational modifications including phosphorylation and myristoylation; it also contains disulfide bonds.
• UniProtKB annotations indicate involvement in host-virus interaction pathways.

Experimental Guidance and Technical Tips

• The immunogen is a recombinant fusion protein spanning amino acids 1–235 of human CHCHD6, covering the N-terminal region, which may be effective for detecting full-length CHCHD6 in Western blotting.
• For WB, a band around 26 kDa is expected; optimize sample preparation and loading conditions to achieve clear resolution.
• This polyclonal antibody is suitable for ELISA; consider pairing with a matched detection antibody or using as a capture reagent for sandwich ELISA after appropriate validation.
• Since the antibody has been tested on human samples, it is recommended to validate cross-reactivity in other species if needed.
• For both applications, titrate the antibody and include positive and negative controls to ensure specificity and minimize background.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. For mitochondrial research and cristae organization studies, we offer a range of antibodies, including this CHCHD6 polyclonal antibody, along with complementary reagents and customized sourcing solutions for rare targets.