Anti-CHCHD4 Polyclonal Antibody (Rabbit pAb) for WB, IHC-P, ELISA - Q8N4Q1

Core Product Specifications and Parameters

Biological Background: CHCHD4 Function and Localization

• CHCHD4, also known as MIA40 or TIMM40, is a mitochondrial intermembrane space import and assembly protein (UniProt Q8N4Q1).
• It functions as the central component of a redox-sensitive import machinery required for the biogenesis of respiratory chain complexes, acting as a chaperone and catalyzing disulfide bond formation in substrate proteins such as COA7, COX17, COX19, MICU1, and TIMCC. Related references: PMID:26004228, PMID:16185709, PMID:19182799, PMID:21059946
• CHCHD4 is essential for the import and oxidative folding of small cysteine-containing proteins (small Tim proteins) in the mitochondrial intermembrane space (IMS). Related references: PMID:16185709, PMID:19182799, PMID:21059946
• The protein localizes to the mitochondrion intermembrane space and is expressed ubiquitously across tissues.
• CHCHD4 operates via a disulfide relay: its oxidized form transiently forms intermolecular disulfide bridges with reduced precursor proteins, oxidizing them and enabling folding; reduced CHCHD4 is then reoxidized by GFER/ERV1. Related references: PMID:23186364
• It mediates disulfide bond formation in MICU1, promoting MICU1-MICU2 heterodimerization essential for mitochondrial calcium uptake regulation. Related references: PMID:26387864
• CHCHD4 is required for the import of COA7 into the IMS. Related references: PMID:30885959
• UniProt keywords include disulfide bond, oxidoreductase, protein transport, and redox-active center.

Experimental Guidance and Technical Tips

• For Western blotting (WB), the predicted molecular weight is approximately 16 kDa; consider using a high-percentage gel and optimize transfer conditions for low-molecular-weight proteins.
• In immunohistochemistry-paraffin (IHC-P), antigen retrieval and tissue fixation conditions may require optimization; endogenous peroxidase quenching is recommended for HRP-based detection.
• This polyclonal antibody may detect isoforms arising from alternative splicing; validation in the relevant sample system is advised.
• Cross-reactivity is reported with human, mouse, and rat samples; for other species, verify sequence homology before use.
• For ELISA applications, pairing with appropriate secondary antibodies and optimizing coating and blocking conditions is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research teams with comprehensive access to IVD raw materials, including validated antibodies, antigens, and ancillary reagents. This CHCHD4 antibody supports research into mitochondrial biology and respiratory chain assembly, areas where CamelBio can source complementary tools such as antibody pairs and optimized detection systems. Whether for small-scale validation or large-volume production, CamelBio connects you with reliable supply solutions to accelerate your diagnostic development.