Anti-ChAT Rabbit Monoclonal Antibody for WB, ELISA - P28329

Core Product Specifications and Parameters

Biological Background: ChAT Function and Localization

• Choline O-acetyltransferase (ChAT) is also known by the aliases CHOACTASE, CMS6, CMS1A, and CMS1A2.
• It catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses, a key step in cholinergic neurotransmission.
• ChAT is highly expressed in cholinergic neurons of the central and peripheral nervous systems and is localized to presynaptic terminals.
• Alternative splicing of the CHAT gene generates multiple transcript variants, potentially influencing enzyme activity or regulation.
• Post‑translational modifications, including phosphorylation, have been identified, though their functional impact remains under investigation.
• Mutations in CHAT are linked to congenital myasthenic syndrome (CMS), a group of disorders characterized by impaired neuromuscular transmission.
• The protein has a predicted molecular weight of approximately 83 kDa, consistent with the product’s validated observation on Western blot.

Experimental Guidance and Technical Tips

• The immunogen sequence (amino acids 649‑748) resides in a region well‑conserved among human, mouse, and rat, explaining the broad cross‑reactivity. Consider this when analyzing results across species.
• For Western blot, run samples under reducing conditions and expect a specific band near 83 kDa. Use a low‑fluorescence PVDF membrane for optimal detection.
• ELISA protocols may employ this antibody as either capture or detection reagent; titrate carefully against recombinant ChAT protein or native tissue lysates.
• Perform blocking with 5% non‑fat milk or BSA in TBST and incubate primary antibody overnight at 4°C for best signal‑to‑noise ratio.
• Always validate the antibody in your specific sample matrix (e.g., brain homogenate, nerve terminal preparations) prior to quantitative assays.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, labs, and research institutes a single destination for IVD raw materials and comprehensive technical support—from concept to clinic. Our portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents, streamlining your path from discovery to commercialization. Alongside this ChAT antibody for cholinergic research, we source rare‑target raw materials and provide consulting services to address every stage of your diagnostic development.