Anti-CHAMP1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q96JM3

Core Product Specifications and Parameters

Biological Background: CHAMP1 Function and Localization

• CHAMP1 (Chromosome alignment-maintaining phosphoprotein 1), also known as Zinc finger protein 828, is a nuclear protein with an apparent molecular weight of ~89 kDa that plays a critical role in mitotic chromosome segregation.
• It is required for proper alignment of chromosomes at the metaphase plate and ensures accurate segregation by maintaining spindle microtubule attachment to kinetochores during sister chromatid biorientation.
• CHAMP1 may recruit the kinetochore-associated proteins CENPE (centromere protein E) and CENPF to the kinetochore, facilitating microtubule–kinetochore interactions.
• The protein localizes to the nucleus and concentrates at the centromere/kinetochore region of chromosomes during mitosis; it is also found associated with the mitotic spindle and cytoplasmic cytoskeleton.
• Post-translational modifications include phosphorylation, acetylation, and ubiquitylation, which likely regulate its mitotic functions.
• CHAMP1 contains a zinc-finger domain, suggesting DNA- or protein-binding capabilities that may contribute to its chromatin- or kinetochore-targeting roles.
• Mutations in CHAMP1 are associated with intellectual disability (MRD40/NEDHILD), underscoring its importance in neurodevelopment and cell division.

Experimental Guidance and Technical Tips

• Since the immunogen encompasses regions 563–812, the antibody likely recognizes both full-length CHAMP1 and any isoforms containing this C-terminal portion; validate specificity in knockout or knockdown systems.
• For Western blot, the predicted molecular weight is 89 kDa; consider running a high-percentage gel (e.g., 8–10%) with adequate transfer time for high-molecular-weight proteins.
• For immunofluorescence/ICC, fixation and permeabilization methods may influence kinetochore/chromosome staining; test methanol or formaldehyde fixation with Triton X-100 permeabilization.
• The antibody cross-reacts with mouse CHAMP1, making it suitable for studies in murine cell lines or tissues; however, sequence alignment in the immunogen region should be verified.
• Perform ELISA titration experiments to determine optimal coating antigen concentration and antibody dilution for sandwich or direct ELISA setups.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, research labs, and institutes with one-stop access to validated IVD raw materials, including optimized monoclonal/polyclonal antibodies, antibody pairs, and bulk ancillary reagents. For targets like CHAMP1 involved in cell division and chromosome biology, we also offer rare-target raw-material sourcing to accelerate your assay development from concept to clinic.