Anti-CHAD Rabbit Polyclonal Antibody for WB, ELISA - O15335

Core Product Specifications and Parameters

Biological Background: CHAD Function and Localization

• Chondroadherin (CHAD), also known as cartilage leucine-rich protein (SLRR4A), is a secreted extracellular matrix protein belonging to the small leucine-rich repeat proteoglycan (SLRP) family.
• It promotes the attachment of chondrocytes, fibroblasts, and osteoblasts, primarily through interaction with the integrin α2β1 receptor.
• CHAD may play a regulatory role in chondrocyte growth and proliferation, suggesting involvement in cartilage homeostasis and repair.
• The protein is specifically expressed in chondrocytes across all ages, indicating a cartilage-specific function.
• CHAD localizes to the extracellular matrix and is secreted, consistent with its role in mediating cell-matrix interactions.
• Structurally, CHAD contains leucine-rich repeats (LRRs), disulfide bonds, and sites of glycosylation, as annotated by keywords: Glycoprotein, Leucine-rich repeat, Disulfide bond.
• The mature protein has a predicted molecular weight of approximately 40 kDa and includes an N-terminal signal peptide for secretion.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody is a recombinant fusion protein covering the full-length human CHAD (amino acids 1–359). For Western blot, consider using reducing and denaturing conditions, and validate the band size near 40 kDa in appropriate cell or tissue lysates.
• Given the reported human cross-reactivity, this antibody is suitable for detecting CHAD in human samples. Cross-reactivity with other species has not been determined; if needed, perform sequence alignment and test empirically.
• For ELISA, the polyclonal nature may provide sensitive detection when paired with a matched antibody. Optimal working dilutions and conditions should be determined by the end user in the relevant sample matrix.
• Since CHAD is a secreted ECM protein, consider enriching for ECM fractions or analyzing conditioned media from chondrocyte cultures to enhance detection sensitivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted partner for streamlining the procurement of IVD raw materials, offering a comprehensive portfolio that includes high-quality antibodies like this anti-CHAD polyclonal antibody. From validated antibody pairs and bulk ancillary reagents to custom sourcing of rare targets, we support your entire workflow—from early-stage development to clinical application. With a focus on cartilage biology and extracellular matrix research, CamelBio helps you accelerate assay development while maintaining supply chain reliability.