Anti-CHAC1 Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - Q9BUX1

Core Product Specifications and Parameters

Biological Background: CHAC1 Function and Localization

• CHAC1 (Glutathione-specific gamma-glutamylcyclotransferase 1), also known as Botch or cation transport regulator-like protein 1, is a 24.4 kDa protein encoded by the CHAC1 gene (Gene ID: 79094).
• It acts as a glutathione-specific gamma-glutamylcyclotransferase, cleaving glutathione into 5-oxo-L-proline and Cys-Gly, with specificity for glutathione over other gamma-glutamyl peptides. Related references: PMID:27913623
• CHAC1 promotes apoptosis by depleting glutathione and functions as a pro-apoptotic component of the unfolded protein response (UPR) pathway, mediating the effects of the ATF4-ATF3-DDIT3/CHOP cascade. Related references: PMID:19109178
• It negatively regulates Notch signaling during embryonic neurogenesis by inhibiting Notch cleavage by furin, maintaining Notch in an immature inactive form, thereby promoting neurogenesis. Related references: PMID:22445366
• Subcellularly, CHAC1 localizes to the cytoplasm, cytosol, and the Golgi apparatus, particularly the trans-Golgi network.
• Keywords: alternative splicing, apoptosis, lyase, neurogenesis, Notch signaling pathway, unfolded protein response.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant protein spanning amino acids 1-222 (full-length CHAC1, NP_077016.3), which may recognize multiple epitopes and is expected to detect endogenous CHAC1.
• Consider using a denaturing SDS-PAGE gel for western blotting, where the predicted molecular weight of CHAC1 is ~24 kDa; validate the band size in your sample system.
• Appropriate positive controls include cell lines or tissues known to express CHAC1 under unfolded protein response conditions (e.g., ER stress induction); include a loading control.
• For immunofluorescence and immunohistochemistry, optimize fixation and permeabilization conditions, and consider antigen retrieval for paraffin-embedded sections.
• Cross-reactivity is reported for human, mouse, and rat; however, pre-testing in your model system is recommended to verify reactivity and minimize non-specific binding.
• ELISA applications may require optimization of coating conditions and antibody dilution; a direct or indirect ELISA format can be explored depending on assay design.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. For research on CHAC1 in apoptosis and neurogenesis, CamelBio can supply the CHAC1 Rabbit pAb along with validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. The company also offers rare target raw-material sourcing to streamline your assay development and procurement needs.