Anti-CGRRF1 Polyclonal Antibody for WB, ELISA - Q99675

Core Product Specifications and Parameters

Biological Background: CGRRF1 Function and Localization

• CGRRF1 (Cell Growth Regulator with RING Finger Domain 1), also known as CGR19 and RNF197, is a RING finger-containing protein implicated in cell growth inhibition.
• Functional studies indicate that CGRRF1 can inhibit growth in several cell lines, suggesting a role in negative regulation of cell proliferation.
• The protein contains a zinc-binding RING finger domain, a characteristic motif often associated with E3 ubiquitin ligase activity.
• Subcellular localization includes the nucleus and endoplasmic reticulum, implying involvement in nuclear processes and possibly ER-related functions.
• CGRRF1 is ubiquitously expressed across tissues, with particularly high expression in testis and the cerebellum, hinting at tissue-specific regulatory roles.
• Post-translational modifications and structural features: The protein is a metal-binding zinc-finger protein, and its 3D structure has been resolved.
• The protein has a molecular weight of approximately 38 kDa (calculated ~38.2 kDa).
• Related keywords include cell cycle and growth arrest, consistent with its proposed function in growth regulation.

Experimental Guidance and Technical Tips

• The immunogen covers a central region (aa 40-170) of CGRRF1, which may be suitable for detecting both full-length protein and potential isoforms lacking terminal epitopes.
• For Western blot, consider using whole-cell lysates from human cell lines with verified CGRRF1 expression, such as those derived from testis or cerebellum tissues, where expression is high.
• ELISA applications may benefit from using a capture-detection format; ensure proper antibody pairing and validation in the relevant sample system.
• Since CGRRF1 localizes to the nucleus and ER, subcellular fractionation could enhance detection specificity in WB.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw-material sourcing partner, supporting diagnostic manufacturers from concept to clinic. This anti-CGRRF1 polyclonal antibody addresses research needs in cell growth regulation and cell cycle mechanisms. Beyond this product, CamelBio offers validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target raw-material sourcing to streamline your assay development.