Anti-CGRP Polyclonal Antibody for WB, IF/ICC, IHC-P, ELISA - P01258 / P06881

Core Product Specifications and Parameters

Biological Background: CGRP (Calcitonin Gene-Related Peptide) Function and Localization

• The CALCA gene undergoes alternative splicing to produce two peptide hormones: calcitonin and calcitonin gene-related peptide (CGRP, also known as CGRP1, CGRP-alpha, CGRP-I). This antibody specifically recognizes the CGRP isoform.
• Calcitonin, encoded by the same gene, is a 32-amino acid peptide hormone that rapidly reduces blood calcium and phosphate levels by promoting their deposition in bones. However, the antibody was raised against the CGRP-specific sequence.
• Calcitonin signaling is mediated through the calcitonin receptor (CALCR) and the CALCR-RAMP2 (AMYR2) receptor complex (PubMed:35324283). Related references: PMID:35324283
• Katacalcin, another cleavage product from the precursor, also exhibits calcium-lowering activity.
• According to UniProt, the protein is secreted and undergoes key post-translational modifications, including amidation, disulfide bond formation, and phosphorylation.
• The immunogen covers residues 49–128 of the prepropeptide, encompassing the CGRP mature region; the antibody is expected to detect both precursor and mature CGRP forms in relevant samples.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning the CGRP pro-region (aa 49–128). For Western blot, bands at ~13 kDa and/or ~15 kDa may be observed, corresponding to precursor forms; mature CGRP (~3.8 kDa) may run at the dye front.
• Consider using low-percentage tricine gels or specialized electrophoresis systems to resolve small peptides if detection of mature CGRP is desired.
• Blocking with BSA rather than milk may reduce background, as CGRP is a small, highly modified peptide.
• For immunohistochemistry and immunofluorescence, perform antigen retrieval suited to the tissue and validate staining in known CGRP-positive tissues (e.g., sensory neurons, cardiovascular tissue).
• Cross-reactivity is reported with mouse and rat; however, endogenous expression levels vary among tissues and disease states, so optimization of sample preparation is advised.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a trusted partner for IVD manufacturers and research laboratories, providing one-stop access to high-quality raw materials, including validated antibody pairs, recombinant proteins, and ancillary reagents. We support the development of assays targeting neuropeptides like CGRP, offering bulk monoclonal/polyclonal antibodies and customized sourcing for rare targets. Whether you need reproducible antibody lots for clinical diagnostics or technical consultation from concept to clinic, CamelBio simplifies your supply chain.