Anti-CGREF1 Rabbit Polyclonal Antibody for WB, ELISA - Q99674

Core Product Specifications and Parameters

Biological Background: CGREF1 Function and Localization

• CGREF1 (Cell growth regulator with EF hand domain protein 1), also known as CGR11 or Hydrophobestin, is a calcium-binding protein characterized by EF-hand domains.
• It mediates calcium-dependent cell-cell adhesion, contributing to tissue organization and integrity.
• Overexpression of CGREF1 inhibits cell growth in multiple cell lines, indicating a role in cell cycle arrest and growth regulation.
• The protein is secreted, consistent with its extracellular adhesive and signaling functions.
• Alternative splicing generates multiple isoforms, and proteomics identification confirms its presence in the human reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to residues 20-200, likely excluding the signal peptide; thus the antibody is expected to recognize the mature form of CGREF1.
• For Western blotting, a band around 33 kDa is anticipated; however, consider potential post-translational modifications that may alter migration.
• In ELISA, use the recombinant protein as a standard and validate the assay under your specific buffer conditions.
• Cross-reactivity with mouse and rat has been reported; it is advisable to verify reactivity in the relevant tissue or cell line before large-scale experiments.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement with a comprehensive portfolio of validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and customized rare-target sourcing. For researchers focusing on cell adhesion and growth regulation, we provide consistent, high-quality tools and supply chain support to accelerate your assay development. Partner with CamelBio to bridge the gap from concept to clinic efficiently.