Anti-CGGBP1 Polyclonal Antibody for WB, ELISA - Q9UFW8

Core Product Specifications and Parameters

Biological Background: CGGBP1 Function and Localization

• CGGBP1 (CGG triplet repeat-binding protein 1) is a DNA-binding protein also known as 20 kDa CGG-binding protein (p20-CGGBP). It belongs to the reference proteome and has been identified by proteomics and direct protein sequencing.
• CGGBP1 specifically binds to nonmethylated 5'-d(CGG)(n)-3' trinucleotide repeats in the FMR1 promoter, and may play a role in regulating FMR1 promoter activity, implicating it in transcription regulation.
• Localized predominantly in the nucleus, consistent with its DNA-binding function.
• Ubiquitously expressed across tissues, with high expression in placenta, thymus, lymph nodes, cerebellum, and cerebral cortex; low expression in other brain regions.
• Post-translational modifications include phosphorylation (phosphoprotein keyword), which may regulate its activity.
• The calculated molecular weight is approximately 18.8 kDa, consistent with the observed 19 kDa band.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–167) of human CGGBP1; consider using full-length or truncated recombinant protein as a positive control.
• For Western blot, a band at approximately 19 kDa is expected; validate detection in your specific sample system using appropriate loading controls.
• For ELISA, the antibody may be used for direct or indirect detection; perform titration to determine optimal working concentration.
• Given nuclear localization, nuclear extraction protocols may enrich the target protein and improve signal.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement for diagnostic manufacturers, providing validated antibodies and bulk reagents. This anti-CGGBP1 polyclonal antibody supports research into FMR1 promoter regulation and trinucleotide repeat disorders. From concept to clinic, rely on CamelBio’s comprehensive sourcing and technical services for your entire project.