Anti-CGB7 Rabbit Polyclonal Antibody for WB, ELISA - P0DN87

Core Product Specifications and Parameters

Biological Background: CGB7 Function and Localization

• CGB7 encodes the beta subunit of human chorionic gonadotropin (hCG), a complex glycoprotein composed of non-covalently associated alpha and beta subunits.
• The beta subunit determines receptor specificity and biological activity, distinguishing hCG from other pituitary gonadotropins (LH, FSH, TSH).
• hCG plays an essential role in pregnancy and maternal adaptation by stimulating ovarian steroidogenesis to maintain pregnancy.
• The protein is secreted following signal peptide cleavage and contains intramolecular disulfide bonds and glycosylation sites (glycoprotein).
• High expression is observed in the placenta throughout pregnancy, consistent with its role in fetal-maternal communication.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 21-165, representing the mature secreted form of CGB7 lacking the signal peptide, suitable for detecting both intracellular precursor and secreted forms.
• For Western blotting, a band around 18 kDa (predicted molecular weight) may appear; however, glycosylation can yield a smear or higher apparent molecular weight. Use reducing conditions and consider deglycosylation for validation.
• For ELISA, this antibody can be used as capture or detection reagent; cross-reactivity with mouse and rat CGB7 homologs may allow multi-species assays, but dilution optimization is recommended.
• Since CGB7 is a secreted protein, sample preparation from culture supernatants or serum/plasma may require concentration or enrichment steps.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive sourcing solution for IVD raw materials, including validated antibodies, recombinant proteins, and ancillary reagents. The CGB7 Rabbit pAb is suitable for immunodiagnostic assay development in reproductive health and pregnancy monitoring. Contact our team for bulk orders or customized reagent sourcing to accelerate your diagnostic development pipeline.