Anti-CGB5 Rabbit Polyclonal Antibody for WB, ELISA - P0DN86

Core Product Specifications and Parameters

Biological Background: CGB5 Function and Localization

• CGB5 encodes the beta-3 subunit of human chorionic gonadotropin (hCG), a glycoprotein hormone composed of non-covalently linked alpha and beta subunits. The alpha subunit is shared with LH, FSH, and TSH, while the beta subunit confers receptor and biological specificity.
• hCG plays an essential role in pregnancy and maternal adaptation by stimulating the ovaries to synthesize steroids that are essential for the maintenance of pregnancy.
• The CGB5 gene is part of a cluster that includes CGB3, CGB5, and CGB8, encoding nearly identical beta subunits resulting from recent gene duplications, which may lead to cross-reactivity among these isoforms.
• The protein is secreted and exhibits high expression in the placenta throughout pregnancy, consistent with its role in maternal-fetal communication.
• Post-translational modifications include N-linked glycosylation and multiple disulfide bonds, critical for proper folding and hormonal activity.
• UniProt keywords classify it as a secreted glycoprotein hormone with alternative splicing isoforms and available 3D-structural data.

Experimental Guidance and Technical Tips

• For Western blot (WB), the predicted molecular weight of CGB5 is approximately 18 kDa; however, glycosylation may cause the protein to migrate at a higher apparent molecular weight. Validate in a relevant sample system (e.g., placental tissue lysate or hCG-expressing cell lines).
• The polyclonal nature of this antibody may result in recognition of other closely related CGB subunits (CGB3 and CGB8) due to high sequence similarity. Consider this when interpreting results across applications.
• In ELISA applications, this antibody can be used to detect hCG in biological fluids such as serum or urine, which is valuable for pregnancy testing and monitoring. Optimize coating and detection conditions for your specific assay format.
• Verify cross-reactivity in other species if needed, as this antibody is validated for human samples only.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive sourcing solution for IVD raw materials, offering validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and specialized support for rare-target acquisitions. This CGB5 antibody supports research in reproductive biology and the development of pregnancy-related diagnostics. We can assist you in sourcing complementary materials for hCG-based assay development, from concept to clinical application.