Anti-CGB Rabbit Polyclonal Antibody for WB, ELISA - P0DN86

Core Product Specifications and Parameters

Biological Background: CGB Function and Localization

• CGB (Choriogonadotropin subunit beta) is the beta subunit of human chorionic gonadotropin (hCG), a glycoprotein hormone composed of non-covalently associated alpha and beta subunits.
• hCG has an essential role in pregnancy and maternal adaptation; it stimulates the ovaries to synthesize steroids necessary for pregnancy maintenance.
• The beta subunits are distinct among the gonadotropin hormones (LH, FSH, TSH) and confer receptor and biological specificity. The CGB gene cluster includes CGB3, CGB5, and CGB8 among others.
• CGB is secreted, consistent with its hormonal role.
• High expression is observed in the placenta throughout pregnancy.
• CGB is a glycoprotein with disulfide bonds, and it undergoes alternative splicing. It is a direct protein sequencing target and a reference proteome component.
• CGB is also of pharmaceutical interest due to its role in pregnancy diagnostics and therapies.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering amino acids 21-165 of human CGB (NP_000728.1). This region corresponds to the mature secreted form, so the antibody is likely to recognize both the precursor and processed forms. For Western blot, detect the target band around 18kDa under reducing conditions; validate the specificity in the relevant sample system (e.g., placental tissue or cell lines expressing hCG).
• For ELISA, consider using the antibody as a capture or detection reagent, and pair with an appropriate alpha subunit antibody if detecting intact hCG. Optimize concentrations based on the assay format and sample type.
• Cross-reactivity with mouse and rat is reported; however, confirm detection in these species by testing appropriate tissue or cell lysates, as sequence homology may vary.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with one-stop access to validated IVD raw materials, including this anti-CGB polyclonal antibody for pregnancy-related hormone detection. Beyond this product, we offer optimized antibody pairs, bulk ancillary reagents, and rare target sourcing services to support your entire diagnostic development workflow. Contact us to streamline your raw-material procurement and accelerate your IVD pipeline.