Anti-cGAS Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - Q8N884

Core Product Specifications and Parameters

Biological Background: cGAS Function and Localization

• cGAS (cyclic GMP-AMP synthase), also known as MB21D1 or C6orf150, is encoded by the CGAS gene. Alternative splicing generates multiple isoforms.
• It functions as a nucleotidyltransferase that catalyzes the synthesis of the cyclic dinucleotide 2'3'-cGAMP from ATP and GTP. Related references: PMID:21478870, PMID:23258413, PMID:23707061, PMID:28214358, PMID:28363908
• cGAS is a pivotal cytosolic DNA sensor that directly binds double-stranded DNA (dsDNA) of sufficient length and curvature, leading to liquid-phase condensation and enzymatic activation. Related references: PMID:28314590, PMID:28363908, PMID:29976794, PMID:30007416
• Upon activation, cGAS-produced 2'3'-cGAMP binds the adaptor STING1, triggering a signaling cascade that induces type-I interferons and other immune mediators. Related references: PMID:28314590, PMID:28363908, PMID:29976794
• cGAS detects dsDNA from a wide range of pathogens including DNA viruses, retroviruses (e.g., HIV-2), and bacteria (e.g., M. tuberculosis), making it a broad innate immune sentinel. Related references: PMID:28363908, PMID:35613581, PMID:23929945, PMID:24269171, PMID:30270045, PMID:26048138
• Under cellular stress (mitochondrial damage, senescence, DNA damage), self-DNA leaks into the cytoplasm or accumulates as micronuclei, aberrantly activating cGAS and driving sterile inflammation. Related references: PMID:28738408, PMID:28759889, PMID:31299200
• cGAS resides predominantly in the cytoplasm but is also found in the nucleus, where its binding to nucleosomes on chromatin inhibits its activity to prevent autoimmune activation. Related references: PMID:31299200, PMID:32911482, PMID:32912999
• Beyond immunity, cGAS modulates DNA damage repair by interacting with PARP1 to suppress homologous recombination and senses translational stress by associating with collided ribosomes. Related references: PMID:30356214, PMID:31544964, PMID:34111399
• Tissue expression is reported in monocytic cell line THP1; post-translational modifications (acetylation, phosphorylation, ubiquitination, palmitoylation) dynamically regulate cGAS localization and activity.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a peptide within the C-terminal region (aa 430–522) of human cGAS. Consider verifying recognition of all splice variants if multiple isoforms are relevant.
• For Western blot (WB), a band of approximately 59 kDa is expected; use appropriate positive controls (e.g., THP1 cell lysate). Validate antibody specificity using cGAS knockout or knockdown samples.
• For immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), optimized antigen retrieval and permeabilization conditions may be required. Include appropriate controls to confirm nuclear/cytoplasmic localization.
• In ELISA applications, ensure the coating buffer and detection conditions are compatible with the rabbit monoclonal format.
• Cross-reactivity has been tested only against human samples; reactivity with other species is not characterized and should be experimentally determined.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with streamlined access to high-quality IVD raw materials, from validated antibodies to recombinant proteins and ancillary reagents. This anti-cGAS rabbit monoclonal antibody is part of our innate immunity portfolio, supporting research into DNA sensing, autoinflammation, and antiviral responses. Our one-stop sourcing model ensures reliable supply in bulk quantities, empowering you to accelerate assay development and clinical translation.